Antisense expression of the Arabidopsis thaliana AtPGIP1 gene reduces polygalacturonase-inhibiting protein accumulation and enhances susceptibility to Botrytis cinerea
Antisense expression of the Arabidopsis thaliana AtPGIP1 gene reduces polygalacturonase-inhibiting protein accumulation and enhances susceptibility to Botrytis cinerea
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DOI：
10.1094/mpmi-19-0931
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发表时间：
2006-08-01
影响因子：
3.5
通讯作者：
De Lorenzo, Giulia
中科院分区：
文献类型：
--
作者：
Ferrari, Simone;Galletti, Roberta;De Lorenzo, Giulia
聚半乳糖醛酸酶（PGs）水解植物细胞壁果胶中的半乳糖醛酸，是几种植物病原真菌的重要毒力因子。在非生物和生物胁迫下，植物积累pg抑制蛋白（PGIPs），降低真菌pg的活性。在拟南芥中，对坏死性真菌灰霉病的重要致病因子BcPG1具有相当活性的PGIPs是由AtPGIP1和AWGIP2两个基因编码的。这两个基因通过不同的信号通路被真菌感染诱导。我们在这里展示了表达反义AtPGIP1基因的转基因拟南芥植物降低了AtPGIP1的抑制活性，并且更容易受到灰孢杆菌的感染。这些结果表明PGIP有助于对该病原体的基础抗性，并强烈支持该蛋白在拟南芥先天免疫中发挥作用的观点。
Polygalacturonases (PGs) hydrolyze the homogalacturonan of plant cell-wall pectin and are important virulence factors of several phytopathogenic fungi. In response to abiotic and biotic stress, plants accumulate PG-inhibiting proteins (PGIPs) that reduce the activity of fungal PGs. In Arabidopsis thaliana, PGIPs with comparable activity against BcPG1, an important pathogenicity factor of the necrotrophic fungus Botrytis cinerea, are encoded by two genes, AtPGIP1 and AWGIP2. Both genes are induced by fungal infection through different signaling pathways. We show here that transgenic Arabidopsis plants expressing an antisense AtPGIP1 gene have reduced AtPGIP1 inhibitory activity and are more susceptible to B. cinerea infection. These results indicate that PGIP contributes to basal resistance to this pathogen and strongly support the vision that this protein plays a role in Arabidopsis innate immunity.