Chemical and physical properties of serum transferrins from several species.
Chemical and physical properties of serum transferrins from several species.
复制标题
几个物种的血清转铁蛋白的化学和物理特性。
DOI：
10.1021/bi00730a005
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发表时间：
1973
期刊：
影响因子：
2.9
通讯作者：
F. Castellino
中科院分区：
文献类型：
--
作者：
B. Hudson;M. Ono;W. J. Brockway;F. Castellino
Billy G. Hudson,* Mikio Ohno, William J. Brockway和Francis J. Castellino摘要：通过沉淀平衡离心测定，牛、兔、马和猪血清中转铁蛋白的分子量分别为77,300,76,700,79,100和76,400。巯基乙醇在6 m盐酸胍中的还原蛋白分子量分别为76,800、77,400、75,400和75,900。这些结果表明，所有四种的转铁蛋白都是由一条多肽链组成的。每个转铁蛋白分子包含两个铁原子。四种转铁蛋白的氨基酸组成相似，但存在显著差异。比较这些糖蛋白的碳水化合物组成揭示
Billy G. Hudson,* Mikio Ohno, William J. Brockway, and Francis J. Castellino abstract: Bovine, rabbit, equine, and porcine serum trans-ferrins have molecular weights of 77,300, 76,700, 79,100, and 76,400, respectively, in dilute aqueous solution as determined by sedimentationequilibrium centrifugation. The mercaptoethanol reduced proteins in 6 m guanidine hydrochloride possess molecular weights of 76,800, 77,400, 75,400, and 75,900, respectively. These results indicatethat transferrin from all four species consists of a single polypeptide chain. Each transferrin bindstwo atoms of iron per molecule. Amino acid compositions of the four species of transferrins are similar but some significant differences exist. Comparison of the carbohydrate composition of these glycoproteins reveals