Sucrose metabolism contributes to in vivo fitness of Streptococcus pneumoniae
Sucrose metabolism contributes to in vivo fitness of Streptococcus pneumoniae
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DOI：
10.1111/j.1365-2958.2007.05878.x
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发表时间：
2007-10-01
影响因子：
3.6
通讯作者：
Camilli, Andrew
中科院分区：
文献类型：
--
作者：
Iyer, Ramkumar;Camilli, Andrew
我们的特点是两个蔗糖代谢系统- sus和scr -和描述他们的作用，在生理和毒性的肺炎链球菌在小鼠模型的运输和肺炎。sus和scr系统分别受Lacl家族阻遏物SusR和ScrR的调节。SusR调节邻近的ABC转运蛋白（susT 1/susT 2/susX）和蔗糖-6-磷酸（S-6-P）水解酶（susH）。ScrR控制邻近的PTS转运蛋白（PTS transporter，PTS T）、果糖激酶（fructokinase，PTS K）和第二S-6-P水解酶（S-6-P hydrolase，S-6-P Hydrolase，S-6-P Hydrolase，S-6-P Hydrolase）。suSH和sus位点突变体在肺中减弱，但在鼻咽携带中减弱。相反地，在肺中表达的cDNAH和scr基因座突变体在鼻咽定殖中减弱。在鼻咽部中，SsuH/SsuH双突变体比SsuH更弱，表明SsuH可以在该生态位中替代。这两种系统都是蔗糖诱导的，ScrH是主要的体外水解酶。该突变体不能在蔗糖上生长，表明sus和scr是S.肺炎。我们提出了一个模型，描述了scr和sus系统的分级调控的推定诱导剂，S-6-P。肺炎定植和疾病。
We characterized two sucrose-metabolizing systems - sus and scr - and describe their roles in the physiology and virulence of Streptococcus pneumoniae in murine models of carriage and pneumonia. The sus and scr systems are regulated by Lacl family repressors SusR and ScrR respectively. SusR regulates an adjacent ABC transporter (susT1/susT2/susX) and sucrose-6-phosphate (S-6-P) hydrolase (susH). ScrR controls an adjacent PTS transporter (scrT), fructokinase (scrK) and second S-6-P hydrolase (scrH)- sus and scr play niche-specific roles in virulence. The susH and sus locus mutants are attenuated in the lung, but dispensable in nasopharyngeal carriage. Conversely, the scrH and scr locus mutants, while dispensable in the lung, are attenuated for nasopharyngeal colonization. The scrH/susH double mutant is more attenuated than scrH in the nasopharynx, indicating SusH can substitute in this niche. Both systems are sucrose-inducible, with ScrH being the major in vitro hydrolase. The scrH/susH mutant does not grow on sucrose indicating that sus and scr are the only sucrose-metabolizing systems in S. pneumoniae. We propose a model describing hierarchical regulation of the scr and sus systems by the putative inducer, S-6-P. The transport and metabolism of sucrose or a related disaccharide thus contributes to S. pneumoniae colonization and disease.