Change in Free Polyamine Contents and Expression Profiles of Two Polyamine Biosynthetic Genes in Citrus Embryogenic Callus under Abiotic Stresses
Change in Free Polyamine Contents and Expression Profiles of Two Polyamine Biosynthetic Genes in Citrus Embryogenic Callus under Abiotic Stresses
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DOI：
10.1080/13102818.2009.10817655
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发表时间：
2009-01
影响因子：
1.4
通讯作者：
J. Wang;J. Liu
中科院分区：
文献类型：
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作者：
J. Wang;J. Liu
柑橘产业发展常常遇到一系列不利的环境因素。多胺在植物对多种非生物胁迫的响应中起着重要作用。以纽荷尔脐橙橙子（Citrus sinensis）胚性愈伤组织为材料，研究了在高盐、低温和高温条件下游离多胺含量的变化以及精氨酸脱羧酶（ADC）和S-腺苷甲硫氨酸脱羧酶（SAMDC）基因的表达。结果表明，在愈伤组织中检测到腐胺、亚精胺和精胺三种多胺。在上述胁迫条件下，3种多胺均有不同程度的积累，其中精胺的积累最为显著。ADC和SAMDC由应激条件诱导。然而，游离多胺含量和这两个基因的表达之间的密切相关性不能完全建立。我们的数据表明，非生物胁迫导致柑橘中游离多胺含量的变化和生物合成基因的转录变化。
ABSTRACT Citrus industry usually encounters a series of adverse environment factors. Polyamines have been shown to play key roles in plant response to a wide range of abiotic stresses. Herein efforts were made to identify the change of free polyamines and expressions of two important polyamine biosynthetic genes, arginine decarboxylase (ADC) and S-adenosylmethionine decarboxylase (SAMDC), in Newhall navel orange (Citrus sinensis) embryogenic callus under high salinity, low and high temperature. Our results showed the three most widely present polyamines, putrescine, spermidine and spermine, could be detected in the callus. Under the aforementioned stresses, the three kinds of polyamines showed accumulation to different extent, in which spermine exhibited the most prominent increase. ADC and SAMDC were induced by the stressful conditions. However, a close correlation between the free polyamine contents and the expression of the two genes could not be fully established. Our data revealed that abiotic stresses led to alteration of free polyamine contents and transcriptional change of the biosynthetic genes in citrus.