The inhibition of collagenase induced degradation of collagen by the galloyl-containing polyphenols tannic acid, epigallocatechin gallate and epicatechin gallate
The inhibition of collagenase induced degradation of collagen by the galloyl-containing polyphenols tannic acid, epigallocatechin gallate and epicatechin gallate
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DOI：
10.1007/s10856-010-4019-3
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发表时间：
2010-05-01
影响因子：
3.7
通讯作者：
Burt, Helen M.
中科院分区：
文献类型：
--
作者：
Jackson, John K.;Zhao, Jinying;Burt, Helen M.
基于胶原的化妆品填充物需要重复处理，这是由于皮内注射部位的填充物的胶原酶衍生降解。本研究的目的是探讨这种降解的抑制作用，由含没食子酰化合物单宁酸，表没食子儿茶素没食子酸酯（EGCG），表儿茶素没食子酸酯（ECG）和没食子酸（GA）。开发了凝胶渗透色谱分析以定量胶原酶诱导的胶原分子量降低。使用HPLC测量化合物与胶原的结合。使用差示扫描量热法（DSC）测量胶原的稳定性。单宁酸，EGCG和ECG（但不是GA）被发现强烈抑制胶原蛋白降解的浓度在低微摩尔范围内。该化合物与胶原和稳定的胶原牢固结合。得出的结论是，鞣酸、表没食子酸没食子酸酯和心电图通过广泛的氢键与胶原蛋白结合，并通过一些疏水性相互作用增强，并阻止胶原酶自由进入胶原蛋白链上的活性位点。
Collagen based cosmetic fillers require repeat treatments due to collagenase derived degradation of the filler in the intradermal injection site. The objective of this study was to investigate the inhibition of this degradation by the galloyl-containing compounds tannic acid, epigallocatechin gallate (EGCG), epicatechin gallate (ECG) and gallic acid (GA). A gel permeation chromatography assay was developed to quantitate the collagenase induced reductions in collagen molecular weight. The binding of the compounds to collagen was measured using HPLC. The stabilization of collagen was measured using Differential Scanning Calorimetry (DSC). Tannic acid, EGCG and ECG (but not GA) were found to strongly inhibit collagen degradation at concentrations in the low micromolar range. The compounds bound strongly to collagen and stabilized collagen. It is concluded that tannic acid, EGCG and ECG bind to collagen via extensive hydrogen bonding augmented by some hydrophobic interactions and prevent the free access of collagenase to active sites on the collagen chains.