Targeted transgenesis through pronuclear injection of improved vectors into in vitro fertilized eggs
Targeted transgenesis through pronuclear injection of improved vectors into in vitro fertilized eggs
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通过将改良载体原核注射到体外受精卵中进行靶向转基因
DOI：
10.1007/s11248-011-9505-y
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发表时间：
2011
影响因子：
3
通讯作者：
H.
中科院分区：
文献类型：
--
作者：
Ohtsuka;M.;Miura;H.;Nakaoka;H.;Kimura;M.;Sato;M.;Inoko;H.
我们最近描述了一种新的转基因技术，称为基于原核注射的靶向转基因（PITT）（Ohtsuka et al. 2010），该技术能够通过受精卵中基于Cre-loxP的重组将转基因靶向整合到预定的基因座（例如Rosa 26）中，从而实现稳定和可重复的转基因表达。
We recently described a new transgenic technique termed pronuclear injection-based targeted transgenesis (PITT)(Ohtsuka et al. 2010), which enables targeted integration of transgenes into predetermined loci (eg Rosa26) by Cre-loxP-based recombination in fertilized eggs, leading to stable and reproducible transgene expression.