Long-term-stable near-infrared polymer dots with ultrasmall size and narrow-band emission for imaging tumor vasculature in vivo.
Long-term-stable near-infrared polymer dots with ultrasmall size and narrow-band emission for imaging tumor vasculature in vivo.
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DOI：
10.1021/acs.bioconjchem.5b00163
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发表时间：
2015-05
影响因子：
4.7
通讯作者：
Liqin Xiong;Fengwen Cao;Xinmin Cao;Yixiao Guo;Yimin Zhang;Xinmei Cai
中科院分区：
文献类型：
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作者：
Liqin Xiong;Fengwen Cao;Xinmin Cao;Yixiao Guo;Yimin Zhang;Xinmei Cai
荧光纳米探针已经成为最有前途的癌症成像材料之一。然而，对于它们在生物系统和环境中的长期胶体稳定性和光稳定性的理解，仍然有许多悬而未决的问题。在这项研究中，我们报道了用于体内肿瘤血管成像的长期稳定的近红外(NIR)聚合物点。采用纳米沉淀法，将近红外染料2，3-萘菁双(三己基硅氧烷)(NIR775)包裹在聚合物点的基质poly[2-methoxy-5-(2-ethylhexyloxy)-1，4-phenylenevinylene]中，制得了具有近红外发光的聚合物点。所制备的近红外聚合物点直径小于5 nm，在778 nm处表现出窄带近红外发射，半峰宽为20 nm，并且在激发峰和发射峰之间有很大的斯托克斯位移(>；300 nm)。此外，无论是人胶质母细胞瘤U87 MG细胞还是人非小细胞肺癌H1299细胞都没有检测到所制备的NIR聚合物DOTS显著摄取。此外，这些近红外聚合物点在4°C的水中至少9个月表现出长期的胶体稳定性和光稳定性，并且能够在静脉注射后显示活体小鼠体内移植的U87 MG肿瘤的血管形成。因此，这些结果为发展基于近红外聚合物点作为荧光纳米探针的小鼠全身成像开辟了新的机会。
Fluorescent nanoprobes have become one of the most promising classes of materials for cancer imaging. However, there remain many unresolved issues with respect to the understanding of their long-term colloidal stability and photostability in both biological systems and the environment. In this study, we report long-term-stable near-infrared (NIR) polymer dots for in vivo tumor vasculature imaging. NIR-emitting polymer dots were prepared by encapsulating an NIR dye, silicon 2,3-naphthalocyanine bis(trihexylsilyloxide) (NIR775), into a matrix of polymer dots, poly[2-methoxy-5-(2-ethylhexyloxy)-1,4-phenylenevinylene] (MEH-PPV), using a nanoscale precipitation method. The prepared NIR polymer dots were sub-5 nm in diameter, exhibited narrow-band NIR emission at 778 nm with a full width at half-maximum of 20 nm, and displayed a large Stokes shift (>300 nm) between the excitation and emission maxima. In addition, no significant uptake of the prepared NIR polymer dots by either human glioblastoma U87MG cells or human non-small cell lung carcinoma H1299 cells was detected. Moreover, these NIR polymer dots showed long-term colloidal stability and photostability in water at 4 °C for at least 9 months, and were able to image vasculature of xenografted U87MG tumors in living mice after intravenous injection. These results thus open new opportunities for the development of whole-body imaging of mice based on NIR polymer dots as fluorescent nanoprobes.