Activation of Human NK Cells by Malaria-Infected Red Blood Cells
Activation of Human NK Cells by Malaria-Infected Red Blood Cells
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DOI：
10.1007/978-1-60761-362-6_29
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发表时间：
2010-01-01
期刊：
影响因子：
--
通讯作者：
Riley, Eleanor M.
中科院分区：
文献类型：
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作者：
Horowitz, Amir;Riley, Eleanor M.
本章描述了一种评估在体外用感染疟疾的红细胞刺激后人类NK细胞激活的方案。通过流式细胞术、细胞表面CD69表达染色和细胞内干扰素-γ的积聚来评估细胞的活化。介绍了恶性疟原虫的体外繁殖和纯化、密度离心法从肝素化血液中分离外周血单核细胞、PBMC的体外培养以及用流式细胞仪对PBMC进行染色和分析的方法。文中给出了一些典型的FACS曲线图的例子。
This chapter describes a protocol to assess activation Of human NK cells following in vitro stimulation with malaria-infected red blood cells. Activation is assessed by flow cytometry, staining for cell surface expression of CD69 and accumulation of intracellular IFN-gamma. Procedures arc described for in vitro propagation and purification of Plasmodium falciparum parasites, separation of peripheral blood mononuclear cells from heparinised blood by density centrifugation, in vitro culture of PBMC and for staining and analysis of PBMC by flow cytometry. Some examples of typical FACS plots arc shown.