A MAJOR FRACTION OF HUMAN INTRAEPITHELIAL LYMPHOCYTES SIMULTANEOUSLY EXPRESSES THE GAMMA/DELTA T-CELL RECEPTOR, THE CD8 ACCESSORY MOLECULE AND PREFERENTIALLY USES THE V-DELTA-1 GENE SEGMENT
A MAJOR FRACTION OF HUMAN INTRAEPITHELIAL LYMPHOCYTES SIMULTANEOUSLY EXPRESSES THE GAMMA/DELTA T-CELL RECEPTOR, THE CD8 ACCESSORY MOLECULE AND PREFERENTIALLY USES THE V-DELTA-1 GENE SEGMENT
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DOI：
10.1002/eji.1830210429
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发表时间：
1991-04-01
影响因子：
5.4
通讯作者：
PFEFFER, K
中科院分区：
文献类型：
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作者：
DEUSCH, K;LULING, F;PFEFFER, K
通过流式细胞术和免疫组织化学研究了大肠粘膜人上皮内淋巴细胞 (IEL) 中 T 细胞受体 (TcR) 类型的频率和可变基因片段表达，并与外周血淋巴细胞 (PBL) 或固有层淋巴细胞 (LPL) 中的频率和可变基因片段表达进行了比较。使用抗 γ/δ TcR 和抗 α/β TcR 单克隆抗体 (mAb)，流式细胞术分析显示，大部分 IEL (37%) 是 γ/δ T 细胞，而在 LPL 和 PBL 中，γ/δ T 细胞只占少数（分别为 4.6% 和 3.8%）。在这些位点，用抗 CD8 mAb 标记的 γ/δ T 细胞数量分别为 58.3% (IEL)、43.3% (LPL) 和 24.4% (PBL)。大肠连续切片的原位染色证实了这些结果。因此，这些数据表明 CD8+ γ/δ T 细胞在人类大肠上皮中选择性积累。此外，对带有 IEL+ 的 gamma/delta TcR 的分析揭示了使用 V-delta-1 基因片段的细胞明显占优势，而 gamma/delta TcR+ PBL 优先表达 V-delta-2。引人注目的是，大多数 V-delta-1+ IEL 的表面都带有 CD8 分子。这些结果被视为大肠上皮中 V-delta-1+ CD8+ γ/δ T 细胞选择性定位的证据。
The frequency of T cell receptor (TcR) type and the variable gene segment expression in human intraepithelial lymphocytes (IEL) from the large intestinal mucosa were studied by flow cytometry and immunohistochemistry, and compared to those in peripheral blood lymphocytes (PBL) - or lamina propria lymphocytes (LPL). Employing anti-gamma/delta TcR and anti-alpha/beta TcR monoclonal antibodies (mAb), flow cytometric analysis revealed that a large fraction of IEL (37%) are gamma/delta T cells, whereas within LPL and PBL gamma/delta T cells comprise a minor population (4.6% and 3.8% respectively). At these sites the number of gamma/delta T cells labeled with anti-CD8 mAb were 58.3% (IEL), 43.3% (LPL) and 24.4% (PBL). In situ staining of serial sections of large intestine confirmed these results. Hence, these data suggest a selective accumulation of CD8+ gamma/delta T cells in the human epithelium of the large intestine. Furthermore, analysis of gamma/delta TcR bearing IEL+ disclosed a marked preponderance of cells using the V-delta-1 gene segment, whereas gamma/delta TcR+ PBL preferentially express V-delta-2. Strikingly, the majority of these V-delta-1+ IEL bear the CD8 molecule on their surface. These results are taken as evidence for a selective localization of V-delta-1+ CD8+ gamma/delta T cells in the epithelium of the large intestine.