Relapsing-remitting multiple sclerosis and human herpesvirus 6 active infection
Relapsing-remitting multiple sclerosis and human herpesvirus 6 active infection
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DOI：
10.1001/archneur.61.10.1523
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发表时间：
2004-10-01
影响因子：
--
通讯作者：
Arroyo, R
中科院分区：
文献类型：
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作者：
Alvarez-Lafuente, R;De las Héras, V;Arroyo, R
背景资料：目的：研究人类疱疹病毒6型（humanherpesvirus 6，HHV-6）与多发性硬化（multiplesclerosis，MS）的关系，并与健康献血员（healthblooddonors，HBDs）进行比较。分别从血清和血液中提取总DNA和信使RNA，采用实时荧光定量逆转录聚合酶链反应（RT-PCR）检测HHV-6的3个即刻早期基因（U16/U17、U89/U90和U94）和两种HHV-6变体结果：16%的RRMS患者检测到活动性HHV-6感染，而HBD患者为0%（P= 0.003）。7例RRMS急性发作患者HHV-6活跃复制，其中1例病毒仍处于潜伏状态。我们没有发现任何统计学显着差异HHV-6活动性或潜伏感染的患者缓解（P= 0.12）。在HHV-6活跃复制的RRMS患者中，急性发作时的病毒载量高于缓解时（P= 0.04）。结论：RRMS患者中存在HHV-6活动性感染，HHV-6活动性复制与复发可能有关;因此，HHV-6活动性感染可能意味着RRMS患者亚组加重的风险更大。
Background: Recent studies have focused on the relationship between human herpesvirus 6 (HHV-6) and multiple sclerosis (MS).Objective: To analyze HHV-6 messenger RNA expression in patients with relapsing-remitting (RR) MS vs healthy blood donors (HBDs).Design: One hundred fifty-four subjects were enrolled in the study: 105 patients with RRMS (32 in relapse) and 49 HBDs. Total DNA and messenger RNA were extracted from serum and blood samples, respectively, and analyzed by quantitative real-time reverse transcription-polymerase chain reaction for the detection of 3 HHV-6 immediate-early genes (U16/U17, U89/U90, and U94) and both HHV-6 variants (HHV-6A and HHV-6B).Results: Active HHV-6 infection was detected in 16% of patients with RRMS vs 0% of HBDs (P=.003). Seven patients with RRMS with exacerbation had HHV-6 active replication, and the virus remained latent in only 1 of them. We did not find any statistically significant difference between HHV-6 active or latent infection for patients in remission (P=.12). Among patients with RRMS with HHV-6 active replication, viral load was higher when they experienced an acute attack than when in remission (P=.04). In those patients with RRMS who had an active infection only, HHV-6A was found. Cell-free HHV-6 DNA detected in serum samples confirmed the results.Conclusions: The results show that a subset of patients with RRMS experience HHV-6 active infection, and there likely is an association between the viral active replication and relapses; therefore, HHV-6 active infection may imply a greater risk of exacerbations in a subgroup of patients with RRMS.