Quantification of Video‐Taped Images in Microcirculation Research Using Inexpensive Imaging Software (Adobe Photoshop)
Quantification of Video‐Taped Images in Microcirculation Research Using Inexpensive Imaging Software (Adobe Photoshop)
复制标题
使用廉价成像软件 (Adobe Photoshop) 对微循环研究中的录像图像进行量化
作者：
J. Brunner;F. Krummenauer;H. Lehr
背景：微循环研究的研究终点通常是录像图像，而不是数字计算机打印出来的。分析这些录像图像以量化微循环参数通常需要基于计算机的图像分析系统。大多数用于图像分析的软件程序都是定制的、昂贵的，并且它们对选定的参数和研究终点的适用性有限。方法和结果：我们在此证明，在带有内置图形采集板的Macintosh G3计算机上运行的廉价的、商业上可用的计算机软件(Adobe Photoshop)为数字化视频图像的量化提供了通用的、易于使用的工具。通过使用活体荧光显微镜从仓鼠皮皱室模型中缺血前后肌肉微循环获得的图像，Photoshop可以简单快速地定量(I)微血管直径，(Ii)功能性毛细血管密度和(Iii)FITC标记的高分子右旋糖苷从毛细血管后微静脉渗漏。我们提出了软件工具的技术准确性和观察者间高度可靠性的证据。结论：廉价的商业成像程序(如Adobe Photoshop)为图像分析提供了多种工具，在微循环研究中具有广泛的潜在应用。
Background: Study end‐points in microcirculation research are usually videotaped images rather than numeric computer print‐outs. Analysis of these videotaped images for the quantification of microcirculatory parameters usually requires computer‐based image analysis systems. Most software programs for image analysis are custom‐made, expensive, and limited in their applicability to selected parameters and study end‐points. Methods and Result: We demonstrate herein that an inexpensive, commercially available computer software (Adobe Photoshop), run on a Macintosh G3 computer with inbuilt graphic capture board provides versatile, easy to use tools for the quantification of digitized video images. Using images obtained by intravital fluorescence microscopy from the pre‐ and postischemic muscle microcirculation in the skinfold chamber model in hamsters, Photoshop allows simple and rapid quantification (i) of microvessel diameters, (ii) of the functional capillary density and (iii) of postischemic leakage of FITC‐labeled high molecular weight dextran from postcapillary venules. We present evidence of the technical accuracy of the software tools and of a high degree of interobserver reliability. Conclusions: Inexpensive commercially available imaging programs (i.e., Adobe Photoshop) provide versatile tools for image analysis with a wide range of potential applications in microcirculation research.