Association of RSV-A ON1 genotype with Increased Pediatric Acute Lower Respiratory Tract Infection in Vietnam.
Association of RSV-A ON1 genotype with Increased Pediatric Acute Lower Respiratory Tract Infection in Vietnam.
复制标题
DOI：
10.1038/srep27856
复制
发表时间：
2016-06-16
影响因子：
4.6
通讯作者：
Yoshida LM
中科院分区：
文献类型：
--
作者：
Yoshihara K;Le MN;Okamoto M;Wadagni AC;Nguyen HA;Toizumi M;Pham E;Suzuki M;Nguyen AT;Oshitani H;Ariyoshi K;Moriuchi H;Hashizume M;Dang DA;Yoshida LM
自2010年在加拿大首次发现RSV-A ON 1以来，ON 1已在全球范围内报告，但有关其临床影响和严重程度的信息一直存在争议。为了研究RSV-A ON 1的临床相关性，研究了2010年1月至2012年12月在越南中部庆和总医院纳入我们基于人群的前瞻性儿科ARI监测的急性呼吸道感染（ARI）病例。收集临床流行病学资料和鼻咽部样本。应用多重PCR方法对13种呼吸道病毒进行了筛查。通过对G-糖蛋白第2高变区进行测序，进一步检测RSV阳性样本的亚组（A/B）和基因型信息。统计分析RSV-A ON 1的临床流行病学特征。共纳入1854例ARI病例，其中426例（23.0%）为RSV阳性。在研究期间，RSV-A和B共循环。在2012年ON 1出现之前，NA 1是RSV-A的主要基因型。RSV相关ARI住院发生率在ON 1出现后明显增加。此外，多因素分析显示，与NA 1病例相比，ON 1病例下呼吸道感染的风险是2.26（95%CI：1.37-3.72）倍，放射学证实的肺炎是1.98（95%CI：1.01-3.87）倍。我们的研究结果表明，ON 1 ARI病例的临床严重程度高于NA 1。
Since the initial discovery of RSV-A ON1 in Canada in 2010, ON1 has been reported worldwide, yet information regarding its clinical impact and severity has been controversial. To investigate the clinical relevance of RSV-A ON1,acute respiratory infection (ARI) cases enrolled to our population-based prospective pediatric ARI surveillance at Khanh Hoa General Hospital, Central Vietnam from January 2010 through December 2012 were studied. Clinical-epidemiological information and nasopharyngeal samples were collected. Multiplex PCR assays were performed for screening 13 respiratory viruses. RSV-positive samples were further tested for subgroups (A/B) and genotypes information by sequencing the G-glycoprotein 2nd hypervariable region. Statistical analysis was performed to evaluate the clinical-epidemiological characteristics of RSV-A ON1. A total of 1854 ARI cases were enrolled and 426 (23.0%) of them were RSV-positive. During the study period, RSV-A and B had been co-circulating. NA1 was the predominant RSV-A genotype until the appearance of ON1 in 2012. RSV-related ARI hospitalization incidence significantly increased after the emergence of ON1. Moreover, multivariate analysis revealed that risk of lower respiratory tract infection was 2.26 (95% CI: 1.37–3.72) times, and radiologically-confirmed pneumonia was 1.98 (95% CI: 1.01–3.87) times greater in ON1 compared to NA1 cases. Our result suggested that ON1 ARI cases were clinically more severe than NA1.