CLONING OF A GLYCINE RECEPTOR SUBTYPE EXPRESSED IN RAT-BRAIN AND SPINAL-CORD DURING A SPECIFIC PERIOD OF NEURONAL DEVELOPMENT
CLONING OF A GLYCINE RECEPTOR SUBTYPE EXPRESSED IN RAT-BRAIN AND SPINAL-CORD DURING A SPECIFIC PERIOD OF NEURONAL DEVELOPMENT
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DOI：
10.1016/0014-5793(91)80383-e
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发表时间：
1991-04-09
期刊：
影响因子：
3.5
通讯作者：
HISHINUMA, F
中科院分区：
文献类型：
--
作者：
AKAGI, H;HIRAI, K;HISHINUMA, F
编码甘氨酸受体 (GlyR) 异构体的互补 (c) DNA 是从用新生大鼠脊髓的 Poly (A) + RNA 在 lambda-ZAPII 中构建的文库中克隆的。 Northern印迹分析表明，与克隆的cDNA杂交的RNA仅在脊髓的胚胎晚期早期出生后阶段才可检测到。 此外，幼鼠的其他中枢神经组织，如海马体和大脑皮层，也富含这种信息。 “新生儿 (N) GlyR”与成人 rad cord 富含的另一种 GlyR 同工型 (AGlyR) 有 71% 同源性。 将NGlyr-cDNA转录的单一RNA注射到非洲爪蟾卵母细胞中诱导甘氨酸门控Cl-通道的功能性形成，然而，其药理学特性与AGlyR不同。
Complementary (c) DNAs encoding a glycine receptor (GlyR) isomer were cloned from libraries constructed in lambda-ZAPII with poly (A) + RNA of neonatal rat spinal cord. Northern blot analysis revealed that RNA hybridized to the cloned cDNA is detectable only for a period of late embryonic early postnatal stage of the spinal cord. Moreover, other central nervous tissues, such as hippocampus and cerebral cortex, in the infant rats are also rich in this message. The 'neonatal (N) GlyR' has 71% homology to that of another GlyR isoform in which adult rad cord is rich (AGlyR). Injection of a single RNA transcribed from the NGlyr-cDNA into Xenopus oocyte induced functional formation of glycine-gated Cl- channels, however, its pharmacological property differed from that of AGlyR.