The use of tri(n-butyl)phosphate detergent mixtures to inactivate hepatitis viruses and human immunodeficiency virus in plasma and plasma's subsequent fractionation.
The use of tri(n-butyl)phosphate detergent mixtures to inactivate hepatitis viruses and human immunodeficiency virus in plasma and plasma's subsequent fractionation.
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使用磷酸三(正丁基)酯洗涤剂混合物灭活血浆中的肝炎病毒和人类免疫缺陷病毒以及血浆的后续分离。
DOI：
10.1046/j.1537-2995.1990.30790385516.x
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发表时间：
1990
期刊：
影响因子：
2.9
通讯作者：
Horowitz,B
中科院分区：
文献类型：
--
作者：
Piët,MP;Chin,S;Prince,AM;Brotman,B;Cundell,AM;Horowitz,B
在血浆采集或合并时用有机溶剂/清洁剂混合物处理血浆可减少技术人员接触感染性病毒，并提高最终产品的病毒安全性。用2%三羟甲基氨基甲烷处理血浆4小时在37°C下的（正丁基）磷酸盐（TNBP），具有1% TNBP和1%聚氧乙烯脱水山梨糖醇单油酸酯（吐温80），或与1%的TNBP和1%的聚氧乙烯醚，（Triton X-45）在30°C下导致≥ 104组织培养感染剂量（TCID 50）的快速和完全灭活水泡性口炎和辛德毕斯病毒，它们被用作替代物。用TNBP和TNBP和Tween-80处理血浆显示出抑制≥ 104 TCID 50的人免疫缺陷病毒。TNBP处理被106黑猩猩感染剂量（CID 50）的B型肝炎病毒和105 CID 50的非甲、非B型肝炎病毒污染的血浆，可预防肝炎向黑猩猩的传播。在用2% TNBP处理血浆后，因子VIII、IX和V以及抗凝血酶III的回收率分别为80%、90%、40%和100%。用TNBP和洗涤剂混合物处理后，所有因素的回收率均≥ 90%。通过标准技术将处理后的血浆分离为抗血友病因子和凝血酶原复合物浓缩物、免疫球蛋白和白蛋白。预先用TNBP或TNBP和洗涤剂处理并不影响所需蛋白质的分离。因此，在执行标准分馏程序之前，似乎有可能消除血浆中的病毒。
The treatment of plasma with organic solvent/detergent mixtures at the time of plasma collection or pooling could reduce the exposure of technical staff to infectious viruses and enhance the viral safety of the final product. Treatment of plasma for 4 hours with 2‐percent tri(n‐ butyl)phosphate (TNBP) at 37°C, with 1‐percent TNBP and 1‐ percent polyoxyethylensorbitan monooleate (Tween 80) at 30°C, or with 1‐percent TNBP and 1‐percent polyoxyethylene ethers, (Triton X‐ 45) at 30°C resulted in the rapid and complete inactivation of ≥ 104tissue culture‐infectious doses (TCID50) of vesicular stomatitis and Sindbis viruses, which are used as surrogates. Treatment of plasma with TNBP and TNBP and Tween‐80 was shown to inactivate ≥ 104TCID50of human immunodeficiency virus. TNBP treatment of plasma contaminated with 106chimpanzee‐infectious doses (CID50) of hepatitis B virus and 105CID50of non‐A, non‐B hepatitis virus prevented the transmission of hepatitis to chimpanzees. Immediately after treatment of plasma with 2‐percent TNBP, the recovery of factors VIII, IX, and V and antithrombin III was 80, 90, 40, and 100 percent, respectively. Recovery of all factors was ≥ 90 percent after treatment with TNBP and detergent mixtures. Treated plasma was fractionated by standard techniques into antihemophilic factor and prothrombin complex concentrates, immune globulin, and albumin. Prior treatment with TNBP or TNBP and detergent did not affect the separations of desired proteins. Therefore, it appears possible to inactivate viruses in plasma before the execution of standard fractionation procedures.