Heterotrophic growth and nutrient productivities of Tetraselmis chuii using glucose as a carbon source under different C/N ratios
Heterotrophic growth and nutrient productivities of Tetraselmis chuii using glucose as a carbon source under different C/N ratios
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不同C/N比下以葡萄糖为碳源的鳝鱼异养生长及养分生产力
DOI：
10.1007/s10811-016-0919-z
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发表时间：
2017-02-01
影响因子：
3.3
通讯作者：
Pan, Kehou
中科院分区：
文献类型：
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作者：
Lu, Lin;Wang, Jun;Pan, Kehou
四藻属包括在水产养殖中广泛用作食用微藻的各种物种。然而，大量培养的四藻属。一直局限于传统的光合自养培养。本文研究了四藻的异养培养条件，并对四藻的生长速率、形态和生物量生产力进行了评价。在异养培养条件下，分析了不同初始C/N比下的蛋白质、多糖、叶绿素、总脂和脂肪酸组成。在严格的异养条件下，朱氏四藻能够以葡萄糖为唯一碳源生长。此外，T.与自养条件相比，在含有10 g L−1葡萄糖的培养基中，chuii显示出更好的异养生长状态。在C/N比为16和12时获得了相对较高的光密度和生物量。干重测定结果表明，T. C/N比为16和12时，叶绿素a的含量分别为1.37 ± 0.04和1.26 ± 0.06 mg mL−1，显著高于自养组。此外，T.在异养条件下，特别是在C/N比为24时，chuii合成更多的多糖。C/N比为4和8的培养条件下，四藻C16：0和C18：1脂肪酸的含量最高，分别为43.36%和47.49%，C/N比为4和24的培养条件下，四藻C14：0、C18：0、C20：2和C20：4脂肪酸的含量最高。这些结果为异养T.通过突出水产养殖中丰富的营养物质，特别是多糖的来源，
The genus Tetraselmis includes various species that are widely used as food microalgae in aquaculture. However, the mass cultivation of Tetraselmis spp. has been restricted to traditional photoautotrophic culture. Here, we investigated the heterotrophic culture condition for Tetraselmis chuii and assessed growth rate, morphology, and biomass productivity of T. chuii under heterotrophic culture conditions. Protein, polysaccharide, chlorophyll, total lipids, and fatty acid compositions under different initial C/N ratios were also analyzed. Tetraselmis chuii was found to be able to grow using glucose as the sole carbon source under strict heterotrophic conditions. Moreover, T. chuii showed a better heterotrophic growth status in the medium with 10 g L−1 glucose compared to the autotrophic condition. Comparatively high optical density and biomass were obtained at C/N ratios of 16 and 12. The dry weights of T. chuii under C/N ratios of 16 and 12 were 1.37 ± 0.04 and 1.26 ± 0.06 mg mL−1, respectively, significantly higher than those of the autotrophic group. In addition, T. chuii synthesized more polysaccharide under heterotrophic conditions, especially at a C/N ratio of 24. Tetraselmis chuii cultured under C/N ratios of 4 and 8 produced the highest percentages of C16:0 (43.36 %) and C18:1 (47.49 %) fatty acids, and more fatty acids, including C14:0, C18:0, C20:2, and C20:4, were detected in the groups with C/N ratios of 4 and 24. These findings may provide the basis for the mass culture of heterotrophic T. chuii by highlighting sources of plentiful nutrient substances, especially polysaccharides, for aquaculture.