Strain-dependent induction of allergic rhinitis without adjuvant in mice
Strain-dependent induction of allergic rhinitis without adjuvant in mice
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DOI：
10.1034/j.1398-9995.1999.00063.x
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发表时间：
1999-06-01
期刊：
影响因子：
12.4
通讯作者：
Harn, DA
中科院分区：
文献类型：
--
作者：
Okano, M;Nishizaki, K;Harn, DA
背景：迄今为止，尚未有报道显示小鼠模型在没有佐剂的情况下局部致敏后诱导抗原特异性 IgE 和鼻嗜酸性粒细胞增多，这是过敏性鼻炎的两个主要标志，这种现象反映了自然暴露。在本报告中，我们试图建立代表过敏性鼻炎起始的小鼠模型。方法：BALB/c、CBA/J 和 C57BL/6 小鼠仅通过鼻内对曼氏血吸虫卵抗原 (SEA) 致敏。重复致敏后，测定血清抗体滴度、鼻腔嗜酸性粒细胞增多和鼻腔淋巴细胞产生的细胞因子。结果：BALB/c小鼠在重复致敏后产生SEA特异性IgE。高剂量 SEA 致敏诱导 CBA/J 小鼠产生 IgE，而 C57BL/6 小鼠在整个观察期间没有显示 IgE 产生，表明 IgE 产生受到遗传调节。 BALB/c 小鼠在鼻刺激后也表现出鼻嗜酸性粒细胞增多。此外，用SEA鼻内致敏的鼻淋巴细胞在体外产生大量的IL-5。结论：这些结果表明，在没有佐剂的情况下，用SEA鼻内致敏可诱导Th2免疫反应，反映了体内和体外过敏性鼻炎起始的标志，这是受基因调控的。
Background: To date, no murine models have been reported to show the induction of both antigen-specific IgE and nasal eosinophilia, two of the major hallmarks of allergic rhinitis, after local sensitization in the absence of adjuvants, a phenomenon which reflects natural exposure. In this report, we attempted to establish a murine model representing an initiation of allergic rhinitis.Methods: BALB/c, CBA/J, and C57BL/6 mice were sensitized intranasally to Schistosoma mansoni egg antigen (SEA) solely. After repeated sensitization, serum Ab titers, nasal eosinophilia, and cytokine production by nasal lymphocytes were determined.Results: BALB/c mice produced SEA-specific IgE after repeated sensitization. High-dose sensitization to SEA induced IgE production in CBA/J mice, while C57BL/6 mice did not show the production throughout the period observed, suggesting that IgE production was regulated genetically. BALB/c mice also exhibited nasal eosinophilia after the nasal challenge. In addition, nasal lymphocytes sensitized with SEA intranasally produced significant amount of IL-5 in vitro.Conclusions: These results suggest that intranasal sensitization with SEA in the absence of adjuvants induces a Th2 immune reaction, reflecting the hallmarks of the initiation of allergic rhinitis both in vivo and in vitro, which is genetically regulated.