Expression of the mitogen-inducible gene-2 (mig-2) is elevated in human uterine leiomyomas but not in leiomyosarcomas
Expression of the mitogen-inducible gene-2 (mig-2) is elevated in human uterine leiomyomas but not in leiomyosarcomas
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DOI：
10.1016/j.humpath.2003.08.019
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发表时间：
2004-01-01
期刊：
影响因子：
3.3
通讯作者：
Konishi, I
中科院分区：
文献类型：
--
作者：
Kato, K;Shiozawa, T;Konishi, I
为了研究最近克隆的细胞增殖相关基因mig-2（丝裂原诱导基因-2）在子宫平滑肌瘤生长中的作用，在正常子宫肌层、平滑肌瘤和子宫平滑肌瘤中检测了该基因在mRNA和蛋白水平的表达。北方印迹、蛋白质印迹和免疫组化染色表明，与正常子宫肌层相比，子宫肌瘤中mig-2的表达增加，尤其是在月经周期的分泌期。与此相反，mig-2的表达在平滑肌瘤中明显降低。对mig-2 cDNA全编码区直接测序和Southern印迹分析显示，在平滑肌瘤和平滑肌肉瘤中均未发现mig-2的突变、重排和扩增。这些结果表明，mig-2的表达在子宫肌瘤中是转录升高的，并可能参与其介导的子宫肌瘤生长。(C)2004年爱思唯尔公司All rights reserved.
To investigate the role of a recently cloned cell proliferation-related gene, mig-2 (mitogen-inducible gene-2), in the growth of uterine leiomyomas, this gene's expression at mRNA and protein levels was examined in normal myometrium, leiomyomas, and leiomyosarcomas of the uterus. Northern blotting, Western blotting, and immunohistochemical staining demonstrated that mig-2 expression was increased in leiomyomas compared with normal myometrium, especially during the secretory phase of the menstrual cycle. In contrast, the mig-2 expression was greatly decreased in leiomyosarcomas. Direct sequencing of the whole coding region of mig-2 cDNA and Southern blotting revealed that mig-2 alterations, such as mutations, rearrangement, and amplification, were not present in either leiomyomas or leiomyosarcomas. These findings suggest that mig-2 expression is transcriptionally elevated in leiomyomas and could be involved in its hormone-mediated growth of leiomyomas of the uterus. (C) 2004 Elsevier Inc. All rights reserved.