The immunoglobulin octanucleotide: independent activity and selective interaction with enhancers.
The immunoglobulin octanucleotide: independent activity and selective interaction with enhancers.
复制标题
免疫球蛋白八核苷酸：独立活性和与增强子的选择性相互作用。
DOI：
10.1126/science.3029871
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Yamamoto,KR
中科院分区：
文献类型：
--
作者：
Parslow,TG;Jones,SD;Bond,B;Yamamoto,KR
单纯疱疹病毒胸苷激酶(Tk)启动子包括一个八核苷酸序列基序(ATTTGCAT)，该基序也是免疫球蛋白kappa基因启动子的重要组成部分。在没有增强子的情况下，含有该元件的tk启动子衍生物比不含该元件的tk启动子衍生物支持更高的转录速率。Kappa增强子的作用增强了B淋巴样细胞中八核苷酸的作用；当这两个元件都存在时，tk启动子的活性增加了一个数量级以上。相反，该启动子中的八核苷酸的存在显著降低了它对非免疫球蛋白增强剂的反应。这些结果表明，八核苷酸可能介导启动子和增强子之间的选择性相互作用。
The thymidine kinase (tk) promoter of herpes simplex virus includes an octanucleotide sequence motif (ATTTGCAT) that is also an essential component of immunoglobulin kappa gene promoters. In the absence of an enhancer,tkpromoter derivatives that contain this element support a higher rate of transcription than those that lack it. The action of the kappa enhancer augments that of the octanucleotide in B lymphoid cells; when both elements are present,tkpromoter activity is increased by more than an order of magnitude. In contrast, the presence of the octanucleotide in this promoter markedly reduces its response to a nonimmunoglobulin enhancer. These results suggest that the octanucleotide may mediate a selective interaction among promoters and enhancers.