HUMAN CYTOTOXIC T-CELLS STIMULATED BY ANTIGEN ON DENDRITIC CELLS RECOGNIZE THE N, SH, F, M, 22K, AND 1B PROTEINS OF RESPIRATORY SYNCYTIAL VIRUS
HUMAN CYTOTOXIC T-CELLS STIMULATED BY ANTIGEN ON DENDRITIC CELLS RECOGNIZE THE N, SH, F, M, 22K, AND 1B PROTEINS OF RESPIRATORY SYNCYTIAL VIRUS
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DOI：
10.1128/jvi.66.4.2102-2110.1992
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发表时间：
1992-04-01
影响因子：
5.4
通讯作者：
OPENSHAW, PJM
中科院分区：
文献类型：
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作者：
CHERRIE, AH;ANDERSON, K;OPENSHAW, PJM
我们检查了 9 名成人对呼吸道合胞 (RS) 病毒 10 种蛋白质中的 9 种的人类细胞毒性 T 细胞库。 在体外用 RS 病毒刺激正常成人的外周血单核细胞。 测试所得多克隆培养物对感染重组牛痘病毒的 B 类淋巴母细胞系的裂解作用，所述重组痘苗病毒表达九种单独的 RS 病毒蛋白中的每一种。 与传统方法相比，使用外周血树突状细胞呈递抗原在更短的培养时间内提供了更容易重现的结果。 最受认可的六种 RS 病毒蛋白是核蛋白 N（九名供体中的九名，其背景裂解率 > 10%；P = 0.0004）、表面蛋白 SH（九名供体中的六名；P = 0.002）和 F（九名供体中的五名；P = 0.008）、基质蛋白 M（九名供体中的五名；P = 0.004）和 22K（九名供体中的三名；P = 0.004）。 = 0.01）和非结构蛋白 1b（九名捐赠者中的六名；P = 0.004）。 对主要表面糖蛋白 G（九个供体中的两个）、内部磷蛋白 P（九个供体之一）或非结构蛋白 1c（九个供体之一）没有显着识别。 识别受到主要组织相容性复合物 I 类限制，但未发现主要组织相容性复合物表型与 T 细胞的蛋白质特异性之间存在关联。 F 和 22K 的识别似乎与最近的感染有关，通过酶联免疫吸附测定检测血清中抗 RS 病毒免疫球蛋白 G 抗体水平升高表明。 由于细胞毒性 T 细胞对 RS 病毒蛋白的识别已被证明对于从感染宿主中清除病毒非常重要，因此 N、M、SH、1b、F 和 22K 蛋白应被视为潜在的疫苗成分。
We examined the human cytotoxic T-cell repertoire of nine adults to 9 of the 10 proteins of respiratory syncytial (RS) virus. Peripheral blood mononuclear cells from normal adults were stimulated with RS virus in vitro. The resulting polyclonal cultures were tested for lysis of B-lymphoblastoid cell lines infected with recombinant vaccinia viruses expressing each of nine individual RS virus proteins. The use of peripheral blood dendritic cells to present antigen gave more easily reproducible results over a shorter culture period than conventional methods. The six RS virus proteins most strongly recognized were the nucleoprotein N (nine of nine donors with > 10% above background lysis; P = 0.0004), the surface proteins SH (six of nine donors; P = 0.002) and F (five of nine donors; P = 0.008), the matrix proteins M (five of nine donors; P = 0.004) and 22K (three of nine donors; P = 0.01) and the nonstructural protein 1b (six of nine donors; P = 0.004). There was no significant recognition of the major surface glycoprotein G (two of nine donors), the internal phosphoprotein P (one of nine donors), or the nonstructural protein 1c (one of nine donors). Recognition was major histocompatibility complex class I restricted, but no association between major histocompatibility complex phenotype and protein specificity of T cells was seen. Recognition of F and 22K appeared to be associated with recent infection indicated by increased levels of anti-RS virus immunoglobulin G antibody in serum measured by enzyme-linked immunosorbent assay. Since cytotoxic T-cell recognition of RS virus proteins has been demonstrated to be important in the clearance of virus from infected hosts, the N, M, SH, 1b, F, and 22K proteins should be considered potential vaccine components.