Proteomics and Metabolomics of Arabidopsis Responses to Perturbation of Glucosinolate Biosynthesis
Proteomics and Metabolomics of Arabidopsis Responses to Perturbation of Glucosinolate Biosynthesis
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DOI：
10.1093/mp/sss034
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发表时间：
2012-09-01
期刊：
影响因子：
27.5
通讯作者：
Chen, Sixue
中科院分区：
文献类型：
--
作者：
Chen, Ya-zhou;Pang, Qiu-Ying;Chen, Sixue
为了了解植物硫代葡萄糖苷代谢的分子网络，利用诱导RNA干扰（RNAi）技术在拟南芥中建立了脂肪族硫代葡萄糖苷生物合成的扰动。选择了两条RNAi线，使用互补的蛋白质组学和代谢组学方法来检测全球蛋白质和代谢物的变化。参与代谢的蛋白质，包括光合作用和激素代谢、蛋白质结合、能量、应激和防御，对硫代葡萄糖苷的扰动表现出明显的响应。与此同时，代谢组学揭示了氨基酸、碳水化合物、肽和激素水平的主要变化。代谢组学数据与蛋白质组学结果相关，揭示了细胞通路/过程与硫代葡萄糖苷代谢之间的密切分子联系。该研究为硫代葡萄糖苷代谢的分子网络提供了一个前所未有的视角，并为硫代葡萄糖苷工程用于提高防御和质量奠定了基础。
To understand plant molecular networks of glucosinolate metabolism, perturbation of aliphatic glucosinolate biosynthesis was established using inducible RNA interference (RNAi) in Arabidopsis. Two RNAi lines were chosen for examining global protein and metabolite changes using complementary proteomics and metabolomics approaches. Proteins involved in metabolism including photosynthesis and hormone metabolism, protein binding, energy, stress, and defense showed marked responses to glucosinolate perturbation. In parallel, metabolomics revealed major changes in the levels of amino acids, carbohydrates, peptides, and hormones. The metabolomics data were correlated with the proteomics results and revealed intimate molecular connections between cellular pathways/processes and glucosinolate metabolism. This study has provided an unprecedented view of the molecular networks of glucosinolate metabolism and laid a foundation towards rationale glucosinolate engineering for enhanced defense and quality.