Retinoic acid-inducible gene-I is induced in endothelial cells by LPS and regulates expression of COX-2
Retinoic acid-inducible gene-I is induced in endothelial cells by LPS and regulates expression of COX-2
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DOI：
10.1006/bbrc.2002.6650
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发表时间：
2002-03-22
影响因子：
3.1
通讯作者：
Satoh, K
中科院分区：
文献类型：
--
作者：
Imaizumi, T;Aratani, S;Satoh, K
细菌脂多糖（LPS）诱导内皮细胞中多种基因的表达，这些基因是各种病理综合征的关键细胞效应物。通过减法杂交鉴定LPS诱导的内皮细胞差异诱导基因，我们发现视黄酸诱导基因I （RIG-1）在LPS刺激的内皮细胞中被诱导。RIG-1编码一种属于DExH-box家族的蛋白，该蛋白在基因表达和细胞功能调控中具有多种作用。环氧合酶-2 （COX-2）也在内皮细胞中被LPS诱导。RIG-1的过表达选择性上调COX-2的表达，并诱导COX-2启动子活性。RIG-1是受刺激内皮细胞中的诱导基因，由于其调节COX-2基因产物表达的能力，可能在血管病理中起重要作用。(C) 2002 Elsevier Science （USA）。
Bacterial lipopolysaccharides (LPS) induce expression of multiple genes in endothelial cells, which are critical cellular effectors in various pathologic syndromes. Using subtractive hybridization to identify genes that are differentially induced in human endothelial cells treated with LPS, we found that retinoic acid-inducible gene I (RIG-1) is induced in endothelial cells stimulated with LPS. RIG-1 encodes a protein belonging to the DExH-box family which has diverse roles in regulation of gene expression and cellular functions. Cyclooxygenase-2 (COX-2) is also induced in endothelial cells by LPS. Overexpression of RIG-1 selectively upregulated expression of COX-2 and also induced COX-2 promoter activity. RIG-1 is an inducible gene in stimulated endothelial cells that may have important roles in vascular pathology by virtue of its ability to regulate expression of the COX-2 gene product. (C) 2002 Elsevier Science (USA).