Isolation and characterization of nanometre aggregates from a Bai-Hu-Tang decoction and their antipyretic effect
Isolation and characterization of nanometre aggregates from a Bai-Hu-Tang decoction and their antipyretic effect
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白虎汤纳米聚集体的分离、表征及其解热作用
DOI：
10.1038/s41598-018-30690-5
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发表时间：
2018-08-15
影响因子：
4.6
通讯作者：
Li, Yongji
中科院分区：
文献类型：
--
作者：
Lu, Shaowa;Su, Hong;Li, Yongji
在中国，汤剂是最常见的临床剂型之一。纳米聚集体（NAs）通常由圆形或不规则的纳米颗粒组成，在以往的煎药研究中已经观察到。白虎汤汤是中国一种古老的临床剂型。本工作的目的是从BHT中分离和表征NAs，并研究其解热作用。采用传统方法制备BHT汤剂。采用高速离心分离法、透射电镜（TEM）和高效液相色谱法（HPLC）研究BHT中聚集体的作用机理和有效成分。除了聚集外，还通过温度测量、酶联免疫吸附测定、细胞摄取测量和荧光成像来评估治疗活性。BHT中大多数NAs的直径在100 nm左右，其球形结构中含有C、O、Mg、Al、Si、Ca、Zn等。聚集体中存在新甘菊素、芒果苷、甘草酸和甘草酸铵等解热活性物质。此外，BHT中的NAs比BHT的其他分散相具有更好的解热作用。特别是白虎汤（N-BHT）的纳米聚集体很容易被细胞吸收，随着时间的推移，NAs的异硫氰酸荧光素（FITC）信号在肺和脑中比在其他器官中更丰富。这些结果表明，解热作用与BHT中的NAs有关。NAs的发现可能为理解BHT煎剂提供一个新的视角，甚至导致中医纳米医学新方法的发展。因此，这一课题值得进一步研究。
In China, a decoction is one of the most common clinical dosage forms. Nanometre aggregates (NAs), which often consist of circular or irregular nanoparticles, have been observed in previous research on decoctions. A Bai-Hu-Tang (BHT) decoction is an ancient clinical dosage form in China. The purpose of this work was to isolate and characterize NAs from BHT and to investigate their antipyretic effect. A BHT decoction was prepared by the traditional method. The mechanism and active components of the aggregates in BHT were investigated by high-speed centrifugation, transmission electron microscopy (TEM), and HPLC (high-performance liquid chromatography). In addition to the aggregation, therapeutic activities were evaluated through temperature measurements, enzyme-linked immunosorbent assays, cellular uptake measurements and fluorescence imaging. The majority of the NAs in BHT had diameters of 100 nm, and the spherical structures contained C, O, Mg, Al, Si, Ca, Zn et al. Antipyretic bioactive compounds, such as neomangiferin, mangiferin, glycyrrhizic acid and ammonium glycyrrhizinate, existed in the aggregates. In addition, the NAs in BHT had a better antipyretic effect than the other dispersion phases of BHT. In particular, the nanometre aggregates of Bai-Hu-Tang (N-BHT) were easily taken up by cells, and the fluorescein isothiocyanate (FITC) signals of NAs were more enriched in the lungs and brain than in other organs over time. These results revealed that the antipyretic effect was associated with the NAs in BHT. The discovery of NAs might present a new perspective for understanding BHT decoctions and even lead to the development of a new nanomedicine approach in traditional Chinese medicine (TCMs). Therefore, this topic deserves further study.