Transcriptome analysis of root transporters reveals participation of multiple gene families in the response to cation stress
Transcriptome analysis of root transporters reveals participation of multiple gene families in the response to cation stress
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DOI：
10.1046/j.1365-313x.2003.01839.x
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发表时间：
2003-09-01
期刊：
影响因子：
7.2
通讯作者：
Amtmann, A
中科院分区：
文献类型：
--
作者：
Maathuis, FJM;Filatov, V;Amtmann, A
植物营养主要取决于膜转运蛋白的活性，膜转运蛋白将矿物质从土壤转运到植物中，并负责矿物质在细胞内和细胞间的分布。大多数植物膜转运蛋白由多基因家族编码，其成员通常表现出重叠的表达模式和高度的序列同源性。此外，许多无机营养素由一个以上的转运蛋白家族转运。这些考虑，再加上大量的迄今未注释的推定转运蛋白基因，阻碍了我们的进展，了解特定的转运蛋白的活动是如何整合到一个响应波动的条件。我们设计了一个寡核苷酸微阵列，代表1096拟南芥转运蛋白基因，并分析了根转运蛋白转录组在96小时内，相对于80毫米NaCl，K+饥饿和Ca 2+饥饿。我们的数据表明，阳离子胁迫导致大多数转运蛋白基因家族中许多基因的转录水平发生变化。跨所有功能组的转运蛋白的转录调控基因的分析显示，如V型ATP酶和水通道蛋白，响应于所有治疗的家庭，和家庭-其中包括假定的非选择性阳离子通道的NaCl治疗和金属转运Ca 2+饥饿条件-响应于特定的离子环境。几个基因家族，包括初级泵，反向转运蛋白和水通道蛋白进行了详细分析，相对于不同亚型的mRNA水平在离子胁迫。聚类分析允许识别不同的表达谱，并发现了几个新的推定的调控基序内的共表达基因组。
Plant nutrition critically depends on the activity of membrane transporters that translocate minerals from the soil into the plant and are responsible for their intra- and intercellular distribution. Most plant membrane transporters are encoded by multigene families whose members often exhibit overlapping expression patterns and a high degree of sequence homology. Furthermore, many inorganic nutrients are transported by more than one transporter family. These considerations, coupled with a large number of so-far non-annotated putative transporter genes, hamper our progress in understanding how the activity of specific transporters is integrated into a response to fluctuating conditions. We designed an oligonucleotide microarray representing 1096 Arabidopsis transporter genes and analysed the root transporter transcriptome over a 96-h period with respect to 80 mm NaCl, K+ starvation and Ca2+ starvation. Our data show that cation stress led to changes in transcript level of many genes across most transporter gene families. Analysis of transcriptionally modulated genes across all functional groups of transporters revealed families such as V-type ATPases and aquaporins that responded to all treatments, and families - which included putative non-selective cation channels for the NaCl treatment and metal transporters for Ca2+ starvation conditions - that responded to specific ionic environments. Several gene families including primary pumps, antiporters and aquaporins were analysed in detail with respect to the mRNA levels of different isoforms during ion stress. Cluster analysis allowed identification of distinct expression profiles, and several novel putative regulatory motifs were discovered within sets of co-expressed genes.