Isolation and characterization of metaldehyde-degrading bacteria from domestic soils.
Isolation and characterization of metaldehyde-degrading bacteria from domestic soils.
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DOI：
10.1111/1751-7915.12719
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发表时间：
2017-11
影响因子：
5.7
通讯作者：
Moir JWB
中科院分区：
文献类型：
--
作者：
Thomas JC;Helgason T;Sinclair CJ;Moir JWB
三聚乙醛是一种常见的杀螺剂，用于控制农业和园艺中的蛞蝓。目前的水处理过程不会破坏它，它在饮用水源中的积累导致饮用水质量经常出现监管失误。为了解决这个问题，我们从国内土壤中分离出了降解四聚乙醛的微生物。培养了两种不同的细菌分离株，它们能够使用四聚乙醛作为唯一的碳源和能源进行原养生长。一个分离株属于不动杆菌属（菌株名称E1），另一个分离株属于Variovorax属（菌株名称E3）。不动杆菌E1能够将四聚乙醛降解至残留浓度< 1 nM，而密切相关的不动杆菌属菌株完全不能降解四聚乙醛。Variovorax E3生长和降解四聚乙醛比不动杆菌E1更慢，并且在Variovorax E3菌株生长结束时残留四聚乙醛。使用这些细菌菌株或允许原位扩增四聚乙醛降解细菌的方法对四聚乙醛进行生物降解，可能代表了处理土壤和水中四聚乙醛污染的一种方法。
Metaldehyde is a common molluscicide, used to control slugs in agriculture and horticulture. It is resistant to breakdown by current water treatment processes, and its accumulation in drinking water sources leads to regular regulatory failures in drinking water quality. To address this problem, we isolated metaldehyde‐degrading microbes from domestic soils. Two distinct bacterial isolates were cultured, that were able to grow prototrophically using metaldehyde as sole carbon and energy source. One isolate belonged to the genus Acinetobacter (strain designation E1) and the other isolate belonged to the genus Variovorax (strain designation E3). Acinetobacter E1 was able to degrade metaldehyde to a residual concentration < 1 nM, whereas closely related Acinetobacter strains were completely unable to degrade metaldehyde. Variovorax E3 grew and degraded metaldehyde more slowly than Acinetobacter E1, and residual metaldehyde remained at the end of growth of the Variovorax E3 strain. Biological degradation of metaldehyde using these bacterial strains or approaches that allow in situ amplification of metaldehyde‐degrading bacteria may represent a way forward for dealing with metaldehyde contamination in soils and water.