Transcriptomic Analysis of Cellular Senescence: One Step Closer to Senescence Atlas.
Transcriptomic Analysis of Cellular Senescence: One Step Closer to Senescence Atlas.
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DOI：
10.14348/molcells.2021.2239
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发表时间：
2021-03-31
影响因子：
3.8
通讯作者：
Kim C
中科院分区：
文献类型：
--
作者：
Kim S;Kim C
在衰老过程中逐渐积累的衰老细胞是衰老的主要原因之一。虽然 senolytics 可以通过特异性消除衰老细胞来改善人类和小鼠的衰老，但 senolytics 的效果在不同的细胞类型中有所不同，这表明衰老存在差异。多种因素可诱发细胞衰老，并且衰老细胞积累的速度因器官而异。此外，由于异质性是由于衰老细胞的时空背景造成的，因此需要体内研究来增加对衰老细胞的了解。由于目前的方法通常无法区分衰老细胞与其他细胞，因此人们正在努力使用批量 RNA 测序来寻找衰老细胞中通常表达的标记。此外，单细胞 RNA (scRNA) 测序可分析每个细胞的转录本，已被用来了解罕见衰老细胞的体内特征。最近，使用该技术的各个物种的每个器官的转录组细胞图谱已发表。在一些器官中发现了不表达先前建立的标记基因的新型衰老细胞。然而，由于scRNA测序技术的通量有限，有关衰老细胞的信息仍然不足。因此，有必要提高scRNA测序技术的通量或开发一种富集稀有衰老细胞的方法。利用快速发展的单细胞技术建立的体内衰老细胞图谱，将通过特异性去除每个组织和个体中的衰老细胞，为精确的返老还童做出贡献。
Senescent cells that gradually accumulate during aging are one of the leading causes of aging. While senolytics can improve aging in humans as well as mice by specifically eliminating senescent cells, the effect of the senolytics varies in different cell types, suggesting variations in senescence. Various factors can induce cellular senescence, and the rate of accumulation of senescent cells differ depending on the organ. In addition, since the heterogeneity is due to the spatiotemporal context of senescent cells, in vivo studies are needed to increase the understanding of senescent cells. Since current methods are often unable to distinguish senescent cells from other cells, efforts are being made to find markers commonly expressed in senescent cells using bulk RNA-sequencing. Moreover, single-cell RNA (scRNA) sequencing, which analyzes the transcripts of each cell, has been utilized to understand the in vivo characteristics of the rare senescent cells. Recently, transcriptomic cell atlases for each organ using this technology have been published in various species. Novel senescent cells that do not express previously established marker genes have been discovered in some organs. However, there is still insufficient information on senescent cells due to the limited throughput of the scRNA sequencing technology. Therefore, it is necessary to improve the throughput of the scRNA sequencing technology or develop a way to enrich the rare senescent cells. The in vivo senescent cell atlas that is established using rapidly developing single-cell technologies will contribute to the precise rejuvenation by specifically removing senescent cells in each tissue and individual.
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影响因子：
64.5
作者：
Burd CE;Sorrentino JA;Clark KS;Darr DB;Krishnamurthy J;Deal AM;Bardeesy N;Castrillon DH;Beach DH;Sharpless NE
通讯作者：
Sharpless NE
影响因子：
64.5
作者：
Enge M;Arda HE;Mignardi M;Beausang J;Bottino R;Kim SK;Quake SR
通讯作者：
Quake SR
影响因子：
14.9
作者：
Casella, Gabriel;Munk, Rachel;Gorospe, Myriam
通讯作者：
Gorospe, Myriam
DOI：
10.1073/pnas.92.20.9363
发表时间：
1995-09-26
影响因子：
11.1
作者：
DIMRI, GP;LEE, XH;CAMPISI, J
通讯作者：
CAMPISI, J
影响因子：
32.4
作者：
Hammond, Timothy R.;Dufort, Connor;Stevens, Beth
通讯作者：
Stevens, Beth