Relationships between regulatory T cells and CD8+effector populations in patients with squamous cell carcinoma of the head and neck
Relationships between regulatory T cells and CD8+effector populations in patients with squamous cell carcinoma of the head and neck
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DOI：
10.1002/hed.20490
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发表时间：
2007-02-01
影响因子：
2.9
通讯作者：
Furuya, Nobuhiko
中科院分区：
文献类型：
--
作者：
Chikamatsu, Kazuaki;Sakakura, Koichi;Furuya, Nobuhiko
背景循环T细胞的稳态以复杂的方式调节，尚未得到很好的定义。癌症患者中1型和2型T细胞亚群之间的平衡被认为调节抗肿瘤免疫。同时，CD 4 + CD 25+调节性T细胞（Treg）作为抗肿瘤免疫反应的有效抑制因子，在维持免疫稳态中也发挥着不可估量的作用。从42名头颈部鳞状细胞癌（SCCHN）患者和24名健康年龄选择的供体中获得外周血。采用流式细胞仪检测T细胞亚群的百分比及其细胞因子表达。尽管SCCHN患者的循环CD 4 + T细胞百分比低于健康供体（p <0.05），但患者中的CD 4 + CD 25+调节性T细胞（Treg）增加（p <0.01）。在用佛波醇12-肉豆蔻酸酯13-乙酸酯/离子霉素离体刺激后，在患者中观察到Th 1和Th 2 CD 4 + T细胞的显著增加。Treg抑制的百分比与总CD 8 + T细胞（p < .05）、CD 8 + IFN-γ +（Tc 1）细胞（p < .05）和CD 8+（L-4+（Tc 2）细胞（p < .01）的百分比相关。在SCCHN患者中Tc 1和Tc 2 CD 8 + T细胞之间存在高度显著的相关性（p <0.0001），但在对照组中没有相关性。Treg在SCCHN患者的循环中成比例增加。这些细胞似乎下调CD 8+效应T细胞的Tc 1和Tc 2亚群中的细胞因子表达，这可能是抗肿瘤反应的原因。(c)2006 Wiley Periodicals，Inc.
Background. Homeostasis of circulating T cells Is regulated in complex ways that have not yet been well defined. The balance between type 1 and type 2 T-cell subsets in cancer patients is thought to modulate antitumor immunity. Meanwhile, CD4+CD25+ regulatory T cells (Treg), which are potent Inhibitors of antitumor immune responses, also play an invaluable role in maintaining immune homeostasis.Methods. Peripheral blood was obtained from 42 patients with squannous cell carcinoma of the head and neck (SCCHN) and 24 healthy age-selected donors. The percentages of T-cell subsets and their cytokine profiles expressed in response to ex vivo stimulation were studied by multicolor flow cytometry.Results. Although patients with SCCHN had a lower percentage (p < .05) of circulating CD4+ T cells than healthy donors, CD4+CD25+ regulatory T cells (Treg) were increased in the patients (p < .01). A significant increase in Th1 and Th2 CD4+ T cells was observed in the patients after ex vivo stimulation with phorbol 12-mynstate 13-acetate/ionomycin. The percent of Treg Inversely correlated with that of total CD8+ T cells (p < .05), CD8+IFN-gamma+ (Tc1) cells (p < .05), and CD8+(L-4+ (Tc2) cells (p < .01). There was a highly significant correlation between Tc1 and Tc2 CD8+ T cells (p < .0001) in SCCHN patients but not in controls.Conclusions. Treg are increased in proportion in the circulation of patients with SCCHN. These cells appear to downregulate cytokine expression in both Tc1 and Tc2 subsets of CD8+ effector T cells, which may be responsible for antitumor responses. (c) 2006 Wiley Periodicals, Inc.