Characterization of the genetic basis of antibiotic resistance in Clostridium difficile.
Characterization of the genetic basis of antibiotic resistance in Clostridium difficile.
复制标题
艰难梭菌抗生素耐药性遗传基础的表征。
DOI：
10.1093/jac/33.3.419
复制
发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Mulligan,ME
中科院分区：
文献类型：
--
作者：
Roberts,MC;McFarland,LV;Mullany,P;Mulligan,ME
用斑点杂交法检测了102株来自美国和其他国家的艰难梭菌，其中69株（68%）与红霉素（Erm）、四环素（泰特）和氯霉素（Cat）耐药基因的9种探针中的至少一种杂交。按频率降序排列的个体决定因素分布为：泰特M，32%;Erm Q，25%;Erm FS，18%;泰特P，15%;泰特K，15%;Cat P，15%;Cat Q，12%;Erm BP，11%;泰特L，7%。这是首次报道Tet P由C. difficile和迄今为止Erm FS仅在Bacteriodes属中发现，而Tet Knor Tet L先前均未在Clostridia属中鉴定。18%的杂交菌株携带编码相同表型的多个决定簇。较高频率的耐药基因与先前暴露于抗菌剂、细胞毒素产生和腹泻有关。从骨髓移植患者中回收的分离株携带的抗生素耐药基因明显少于免疫功能正常的普通药物患者。然而，这可能是由于每个都位于不同的地点。抗生素耐药决定因子可能在与C.很难
By using dot blot hybridization, 69 of 102Clostridium difficileisolates (68%) from the United States and other countries hybridized with at least one of nine DNA probes for erythromycin (Erm), tetracycline (Tet) or chloramphenicol (Cat) resistance determinants. The distribution of individual determinants in descending order of frequency was:Tet M, 32%;Erm Q, 25%;Erm FS, 18%;Tet P, 15%;Tet K, 15%;Cat P, 15%;Cat Q, 12%;Erm BP, 11%;Tet L, 7%. This is the first report ofTet Pbeing carried byC. difficileand hithertoErm FShas only been found within the genusBacteriodes, while neitherTet KnorTet Lhave been previously identified among the genusClostridia. Eighteen percent of the hybridizing isolates carried multiple determinants coding for the same phenotype. A higher frequency of resistance genes was associated with prior exposure to antimicrobial agents, cytotoxin production and diarrhoea. Isolates recovered from bone marrow transplant patients carried significantly fewer antibiotic resistance genes than did those from immunocompetent general medicine patients. However, this may be due to the fact that each was located at a different site. Antibiotic resistance determinants may play a role in the virulence associated withC. difficile.