Limiting dilution analysis of murine epidermal stem cells using an in vivo regeneration assay.
Limiting dilution analysis of murine epidermal stem cells using an in vivo regeneration assay.
复制标题
使用体内再生测定对小鼠表皮干细胞进行有限稀释分析。
DOI：
10.1007/978-1-60761-380-0_29
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Ghadially,Ruby
中科院分区：
文献类型：
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作者：
Strachan,LaurenR;Ghadially,Ruby
表皮干细胞在组织动态平衡、创面修复、肿瘤启动和基因治疗等方面具有重要作用。在这里，我们描述了一种体内再生试验，以测试角质形成细胞前体细胞在体内长期维持表皮的能力。在连续时间点的体内再生试验中，对表皮再生单位的极限稀释分析允许量化短期(转送扩增细胞)和长期(干细胞)再生细胞的频率。
Epidermal stem cells are of major importance for tissue homeostasis, wound repair, tumor initiation, and gene therapy. Here we describe an in vivo regeneration assay to test for the ability of keratinocyte progenitors to maintain an epidermis over the long-term in vivo. Limiting dilution analysis of epidermal repopulating units in this in vivo regeneration assay at sequential time points allows the frequency of short-term (transit amplifying cell) and long-term (stem cell) repopulating cells to be quantified.