Acute sprint exercise transcriptome in human skeletal muscle
Acute sprint exercise transcriptome in human skeletal muscle
复制标题
DOI：
10.1371/journal.pone.0223024
复制
发表时间：
2019-10-24
期刊：
影响因子：
3.7
通讯作者：
Jansson, Eva
中科院分区：
文献类型：
--
作者：
Rundqvist, Hakan Claes;Montelius, Andreas;Jansson, Eva
AimTo研究全球基因表达响应深刻的代谢和激素应激诱导急性sprint exercise.MethodsHealthy男性和女性（n = 14）进行了三个全面的周期冲刺穿插20分钟的恢复。在第一次冲刺前、最后一次冲刺后2小时和20分钟进行肌肉活检。微阵列分析进行分析急性基因表达反应和重复bloodsamples.ResultsIn骨骼肌，一组立即早期基因，FOS，NR 4A 3，MAFF，EGR 1，JUNB后，短跑运动显着上调。从879个差异表达基因的基因本体论分析揭示了各种上游调节因子和下游生物功能的预测激活。基因标签预测了短跑运动后骨骼肌质量的转换增强，并提出了一些新的诱导基因，如WNT 9A，FZD 7和KLHL 40。短跑运动后，循环游离脂肪酸（FFA）显著增加，与PGC-1A和下游基因PERM 1的上调和预测脂质周转增强的基因特征平行。增加生长激素和胰岛素在血液中的基因表达的变化，这两种激素被预测为上游regulator.Conclusionis的第一项研究报告的全球基因表达在骨骼肌急性短跑运动和几个新的发现。首先，与肌肉肥大不是一段时间的短跑训练后的典型发现一致，肥大和萎缩因素都受到调节。第二，全身游离脂肪酸和激素和暴露可能参与了短跑运动引起的基因表达的变化。
AimTo examine global gene expression response to profound metabolic and hormonal stress induced by acute sprint exercise.MethodsHealthy men and women (n = 14) performed three all-out cycle sprints interspersed by 20 min recovery. Muscle biopsies were obtained before the first, and 2h and 20 min after last sprint. Microarray analysis was performed to analyse acute gene expression response and repeated blood samples were obtained.ResultsIn skeletal muscle, a set of immediate early genes, FOS, NR4A3, MAFF, EGR1, JUNB were markedly upregulated after sprint exercise. Gene ontology analysis from 879 differentially expressed genes revealed predicted activation of various upstream regulators and downstream biofunctions. Gene signatures predicted an enhanced turnover of skeletal muscle mass after sprint exercise and some novel induced genes such as WNT9A, FZD7 and KLHL40 were presented. A substantial increase in circulating free fatty acids (FFA) was noted after sprint exercise, in parallel with upregulation of PGC-1A and the downstream gene PERM1 and gene signatures predicting enhanced lipid turnover. Increase in growth hormone and insulin in blood were related to changes in gene expressions and both hormones were predicted as upstream regulators.Conclusionis the first study reporting global gene expression in skeletal muscle in response to acute sprint exercise and several novel findings are presented. First, in line with that muscle hypertrophy is not a typical finding after a period of sprint training, both hypertrophy and atrophy factors were regulated. Second, systemic FFA and hormonal and exposure might be involved in the sprint exercise-induced changes in gene expression.