THE REGULATOR OF STREPTOMYCIN GENE-EXPRESSION, STRR, OF STREPTOMYCES-GRISEUS IS A DNA-BINDING ACTIVATOR PROTEIN WITH MULTIPLE RECOGNITION SITES
THE REGULATOR OF STREPTOMYCIN GENE-EXPRESSION, STRR, OF STREPTOMYCES-GRISEUS IS A DNA-BINDING ACTIVATOR PROTEIN WITH MULTIPLE RECOGNITION SITES
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DOI：
10.1111/j.1365-2958.1995.mmi_18010151.x
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发表时间：
1995-10-01
影响因子：
3.6
通讯作者：
DISTLER, J
中科院分区：
文献类型：
--
作者：
RETZLAFF, L;DISTLER, J
在灰色链霉菌中，至少一个链霉素生物合成基因strB1的表达依赖于途径特异性激活蛋白STRR。我们在这里证明了STRR是一种DNA结合蛋白，它与strB1启动子片段特异地相互作用。足迹实验表明，STRR蛋白与位于strB1启动子上游的反向重复序列结合。通过序列比较、凝胶滞留和足迹研究，在灰链霉菌和灰链霉菌的STR-STS基因簇中进一步鉴定出具有共同序列GTTCGActG(N)(11)CagTcGAAc的STRR结合位点。遗传和生化证据有力地支持了STRR蛋白通过与灰链霉菌和灰链霉菌STR-STS基因簇中的多个结合位点相互作用来激活链霉素生物合成基因表达的模型。
In Streptomyces griseus the expression of at least one streptomycin biosynthetic gene, strB1, is dependent on the pathway-specific activator protein StrR. We show here that StrR is a DNA-binding protein which specifically interacts with the strB1 promoter fragment. Footprinting experiments demonstrate that the StrR protein binds to an inverted repeat located upstream of the strB1 promoter. Further StrR-binding sites having the consensus sequence GTTCGActG(N)(11)CagTcGAAc were identified in the str-sts gene clusters of S. griseus and Streptomyces glaucescens by sequence comparison, gel retardation, and footprinting studies. The genetic and biochemical evidence strongly supports the model of the StrR protein activating the expression of streptomycin biosynthetic genes by interacting with multiple binding sites within the str-sts gene clusters of S. griseus and S. glaucescens.