Alteration of Fungal Microbiota After 5-ASA Treatment in UC Patient
Alteration of Fungal Microbiota After 5-ASA Treatment in UC Patient
复制标题
UC 患者 5-ASA 治疗后真菌微生物群的变化
DOI：
10.1093/ibd/izz207
复制
发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Liu Yulan
中科院分区：
文献类型：
--
作者：
Xu Jun;Chen Ning;Song Yang;Wu Zhe;Wu Na;Zhang Yifan;Ren Xinhua;Liu Yulan
治疗方案对溃疡性结肠炎（UC）患者真菌菌群的影响尚不清楚。在这里，我们旨在阐明5-氨基水杨酸（5-ASA）治疗对UC患者肠道真菌微生物群的影响。57名UC患者，包括20名treatment-naïve和37名5- asa治疗，被招募到一项探索性研究中。我们使用its1 -2 rdnassequencing比较了这两组患者的肠道真菌谱。20名treatment-naïve UC患者中有10名接受了随访，并参加了一项验证研究，并接受了5-ASA治疗。我们评估了这些患者在5-ASA治疗前后真菌菌群的纵向差异。验证研究的结果与勘探研究的结果一致。在非炎症和炎症粘膜中，子囊菌是优势门。在门水平上，5-ASA治疗前炎症粘膜内子囊菌群减少。在属水平上，在炎症粘膜中，胞杆菌、羊肠菌和拟青霉等病原体增加，腐殖质菌和维克菌减少。在5-ASA治疗后，在非炎症和炎症粘膜中，子囊菌群和wickerhaomyia增加，而镰刀菌群、镰刀菌群、羊肠菌群和拟青霉群减少。此外，炎症粘膜中平衡的细菌-真菌相关性被中断，5-ASA治疗改变了UC患者群体特异性真菌微生物群并恢复了细菌-真菌相关性。我们的研究结果表明，在5-ASA治疗后，炎症粘膜中真菌的多样性和组成发生了变化，细菌-真菌的相关性得以恢复。
The effect of treatment regimens on fungal microbiota is unclear in ulcerative colitis (UC) patients. Here, we aimed to clarify the effect of 5-aminosalicylic acid (5-ASA) treatment on gut fungal microbiota in UC patients. Fifty-seven UC patients, including 20 treatment-naïve and 37 5-ASA-treated, were recruited into an exploration study. We compared the gut fungal profiles of these 2 groups of patients usingITS1-2 rDNAsequencing. Ten out of 20 treatment-naïve UC patients were followed up and enrolled for a validation study and underwent a 5-ASA treatment. We assessed the longitudinal differences of fungal microbiota in these patients before and after 5-ASA treatment. Results acquired from the validation study were accordant to those from the exploration study. Ascomycota was the dominant phylum in both noninflamed and inflamed mucosae. At the phylum level, Ascomycota decreased in inflamed mucosae before 5-ASA treatment. At the genus level, pathogens such asScytalidium,Morchella, andPaecilomycesincreased, andHumicolaandWickerhamomycesdecreased in inflamed mucosae. After 5-ASA treatment, Ascomycota andWickerhamomycesincreased andScytalidium,Fusarium,Morchella, andPaecilomycesdecreased in both noninflamed and inflamed mucosae. Additionally, the balanced bacteria–fungi correlation was interrupted in inflamed mucosae, and 5-ASA treatment altered group-specific fungal microbiota and restored bacteria–fungi correlation in UC patients. Our results demonstrated that fungal diversity and composition were altered and the bacteria–fungi correlation was restored in inflamed mucosae after 5-ASA treatment.