ELISA for human serum leucine-rich alpha-2-glycoprotein-1 employing cytochrome c as the capturing ligand.
ELISA for human serum leucine-rich alpha-2-glycoprotein-1 employing cytochrome c as the capturing ligand.
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ELISA用于富含细胞色素c的人血清含有富含浅亮氨酸的α-2-糖蛋白-1作为捕获的配体。
DOI：
10.1016/j.jim.2008.03.004
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发表时间：
2008-07-20
影响因子：
2.2
通讯作者：
Jemmerson R
中科院分区：
文献类型：
--
作者：
Weivoda S;Andersen JD;Skogen A;Schlievert PM;Fontana D;Schacker T;Tuite P;Dubinsky JM;Jemmerson R
富含亮氨酸的α-2-糖蛋白-1（LRG）是一种功能未知的血清糖蛋白，基于定性评估，其有望作为某些疾病（包括微生物感染和癌症）的生物标志物。然而，缺乏定量测定LRG限制了其应用。本文描述了一种用于定量人血清中LRG的间接酶联免疫吸附测定法（ELISA），其中采用细胞色素c作为捕获配体，并使用对LRG具有特异性的单克隆抗体来检测捕获的糖蛋白。应用此方法定量LRG在各种患者的血清中被证明。通过本试验测定的对照受试者血清中LRG的浓度约为50 μg/ml。与已发表报告的预期一致，发现在一些细菌感染（中毒性休克综合征，TSS）患者的血清中LRG显著升高。与未感染的对照受试者相比，在感染人类免疫缺陷病毒的患者中LRG仅略微升高，而在非感染性疾病（炎性关节炎和神经系统疾病，主要是帕金森病）患者中观察到正常水平的LRG。虽然LRG和C-反应蛋白（CRP）都是由肝脏产生的，并被归类为急性时相蛋白，但患者血清中LRG和CRP水平之间没有显著相关性。因此，LRG和CRP测量是非冗余的，并且指示不同的生理环境。本报告中描述的ELISA应有助于进一步评估血清LRG作为临床诊断的生物标志物。
Leucine-rich alpha-2-glycoprotein-1 (LRG) is a serum glycoprotein of unknown function that has shown promise based on qualitative assessments as a biomarker for certain diseases including microbial infections and cancer. However, the lack of a quantitative assay for LRG has limited its application. Here an indirect enzyme-linked immunosorbent assay (ELISA) for quantifying LRG in human serum is described in which cytochrome c is employed as the capturing ligand and a monoclonal antibody specific for LRG is used to detect the captured glycoprotein. Application of this assay in quantifying LRG in various patients' sera is demonstrated. The concentration of LRG in sera of control subjects as determined by this assay is approximately 50 μg/ml. Consistent with expectations from published reports, LRG was found to be significantly elevated in the sera of some patients with a bacterial infection (toxic shock syndrome, TSS). LRG was only slightly elevated in patients infected with the human immunodeficiency virus as compared to uninfected control subjects, while normal levels of LRG were observed in patients with non-infectious diseases (inflammatory arthritis and neurological disorders, primarily Parkinson's disease). Although LRG and C-reactive protein (CRP) are both produced by the liver and are classified as acute-phase proteins, there was no significant correlation between the levels of LRG and CRP in the sera of the patients. Thus, LRG and CRP measurements are non-redundant and indicate different physiological contexts. The ELISA described in this report should be useful to further assess serum LRG as a biomarker for clinical diagnostics.
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DOI：
10.1515/bchm2.1977.358.1.639
发表时间：
1977-01-01
影响因子：
--
作者：
HAUPT, H;BAUDNER, S
通讯作者：
BAUDNER, S
影响因子：
2.9
作者：
Bini, L;Magi, B;Pallini, V
通讯作者：
Pallini, V
影响因子：
7.2
作者：
Cummings, Chad;Walder, Jennifer;Jemmerson, Ronald
通讯作者：
Jemmerson, Ronald
DOI：
10.1016/j.jchromb.2007.01.029
发表时间：
2007-06-01
期刊：
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
影响因子：
--
作者：
Kakisaka T;Kondo T;Okano T;Fujii K;Honda K;Endo M;Tsuchida A;Aoki T;Itoi T;Moriyasu F;Yamada T;Kato H;Nishimura T;Todo S;Hirohashi S
通讯作者：
Hirohashi S
DOI：
10.1073/pnas.84.24.9180
发表时间：
1987-12-01
影响因子：
11.1
作者：
JEMMERSON, R
通讯作者：
JEMMERSON, R