CLONING AND STRUCTURAL-ANALYSIS OF ALPHA-LATROINSECTOTOXIN CDNA - ABUNDANCE OF ANKYRIN-LIKE REPEATS
CLONING AND STRUCTURAL-ANALYSIS OF ALPHA-LATROINSECTOTOXIN CDNA - ABUNDANCE OF ANKYRIN-LIKE REPEATS
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DOI：
10.1111/j.1432-1033.1993.tb17741.x
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发表时间：
1993-04-01
期刊：
影响因子：
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通讯作者：
GRISHIN, E
中科院分区：
文献类型：
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作者：
KIYATKIN, N;DULUBOVA, I;GRISHIN, E
α-latroinsectotoxin（alpha-LIT）是一种只对昆虫有选择性的突触前神经毒素，是从黑寡妇蜘蛛Latrodectus mactans tredecimguttatus的毒腺中提取的。从蜘蛛毒腺cDNA文库中分离编码推定的α-LIT前体的cDNA。该cDNA含有4236个碱基对的开放阅读框架，对应于由1411个氨基酸组成的157826-Da蛋白。成熟的α-LIT分子量约为130 kDa，可能来源于初级翻译产物的N-末端和C-末端区域的双重加工。在残基464-1176上延伸的结构区几乎完全由锚定样重复序列组成，其代表也在α-latrotoxin（α-LTX）中发现的基序，其对脊椎动物具有选择性作用。α-LIT和α-LTX氨基酸序列的总比对揭示了34.1%的总体相似性。在位于α-LIT和α-LTX的锚定重复结构域内的类似的富含半胱氨酸的区域中观察到强序列趋异。
Alpha-latroinsectotoxin (alpha-LIT), purified from venom glands of the black widow spider Latrodectus mactans tredecimguttatus, is a presynaptic neurotoxin selective only for insects. A cDNA encoding the putative alpha-LIT precursor was isolated from a spider venom gland cDNA library. The cDNA contains a 4236-base-pair open reading frame corresponding to a 157826-Da protein composed of 1411 amino acids. The mature alpha-LIT, with molecular mass approximately 130 kDa, is probably derived from double processing in the N-terminal and C-terminal regions of the primary translation product. The structure region, extending over residues 464-1176, is composed almost entirely of ankyrin-like repeats which represent a motif also found in the alpha-latrotoxin (alpha-LTX), which has selective action on vertebrates. Total alignment of the alpha-LIT and alpha-LTX amino acid sequences reveals an overall similarity of 34.1 %. Strong sequence divergence is observed in analogous cysteine-rich regions situated within the ankyrin-repeat domains of both alpha-LIT and alpha-LTX.