Trichophyton tonsurans exocellular protease expression: correlation with clinical presentation in tinea capitis.
Trichophyton tonsurans exocellular protease expression: correlation with clinical presentation in tinea capitis.
复制标题
断发毛癣菌外细胞蛋白酶表达：与头癣临床表现的相关性。
DOI：
10.1046/j.1365-2230.2002.01602.x
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发表时间：
2002
影响因子：
4.1
通讯作者：
Abdel-Rahman,SM
中科院分区：
文献类型：
--
作者：
Abdel-Rahman,SM
总结头癣仍然是一个压倒性的流行病的儿童。尽管事实上，它被描述了超过世纪前，疾病的发病机制仍然不完全的特点。本研究旨在评估临床断端毛癣菌分离物中真菌蛋白酶表达的菌株间变异性是否与疾病严重程度相关。入组了患有头癣的儿童，并通过在4分量表上对8种症状进行分级来确定所有受试者的临床严重程度评分（CSS）。通过刷培养收集真菌样本，置于水性培养基中并在32 °C下孵育5天。将培养物上清液冻干，并将等分试样用于表征蛋白酶活性。胶原酶、弹性蛋白酶和角蛋白酶的酶活性（标准化为总可溶性蛋白）分别变化550倍、150倍和6倍。随着感染时间的延长，弹性蛋白酶和胶原酶活性显着降低。在一半的儿童中，CSS直接响应于胶原酶和弹性蛋白酶的产生而增加，而在其余儿童中，CSS与酶活性无关。酶活性和病程之间的关系与皮肤真菌病中酶调节的理论是一致的;然而，发现两个潜在的儿童亚群对真菌抗原存在不同的反应还有待描述。
SummaryTinea capitis remains an overwhelmingly prevalent disease in children. Despite the fact that it was described over a century ago, disease pathogenesis remains incompletely characterized. This investigation was designed to evaluate whether inter‐strain variability in fungal protease expression for clinicalTrichophyton tonsuransisolates correlates with disease severity. Children with tinea capitis were enrolled and a clinical severity score (CSS) determined for all subjects by grading eight symptoms on a 4‐point scale. Fungal specimens were collected by brush culture, placed in aqueous medium and incubated at 32 °C for 5 days. The culture supernatant was lyophilized and aliquots used to characterize protease activity. Enzyme activity, normalized to total soluble protein, varied 550‐fold, 150‐fold and 6‐fold for collagenase, elastase and keratinase, respectively. A significant decrease in elastase and collagenase activity was observed with increasing duration of infection. In one‐half of the children, CSS increased in direct response to collagenase and elastase production, while CSS was independent of enzyme activity in the remaining children. The relationship between enzyme activity and time course of disease are consistent with theories on enzyme regulation in dermatophytoses; however, the finding that two potential subsets of children exist with varied response to fungal antigens has yet to be described.