A SPECIALIZED PATHWAY AFFECTING VIRULENCE GLYCOCONJUGATES OF LEISHMANIA
A SPECIALIZED PATHWAY AFFECTING VIRULENCE GLYCOCONJUGATES OF LEISHMANIA
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DOI：
10.1126/science.7569927
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发表时间：
1995-09-29
期刊：
影响因子：
56.9
通讯作者：
BEVERLEY, SM
中科院分区：
文献类型：
--
作者：
DESCOTEAUX, A;LUO, Y;BEVERLEY, SM
利什曼原虫必须在细胞表面组装一种复杂的糖脂，即脂磷酸聚糖（LPG），才能产生毒力和传播。功能互补鉴定了基因LPG 2，其编码与LPG生物合成的细胞内区室化有关的完整高尔基体膜蛋白。ipg 2（-）突变体仅缺乏特征性的磷酸二氢甘油酯重复序列，通常存在于LPG和其他被认为对感染性至关重要的表面或分泌分子上。与此相反，一个相关的酵母基因，VAN 2/VRG 4，是必要的，需要一般高尔基体功能。这些结果表明，LPG 2参与了一个专门的毒力途径，这可能提供了一个有吸引力的化疗靶点。
For virulence and transmission, the protozoan Leishmania must assemble a complex glycolipid on the cell surface, the lipophosphoglycan (LPG). Functional complementation identified the gene LPG2, which encodes an integral Golgi membrane protein implicated in intracellular compartmentalization of LPG biosynthesis. Ipg2(-) mutants lack only characteristic disaccharide-phosphate repeats, normally present on both LPG and other surface or secreted molecules considered critical for infectivity. In contrast, a related yeast gene, VAN2/VRG4, is essential and required for general Golgi function. These results suggest that LPG2 participates in a specialized virulence pathway, which may offer an attractive target for chemotherapy.