Monoamine oxidases of the human brain and liver.
Monoamine oxidases of the human brain and liver.
复制标题
人脑和肝脏的单胺氧化酶。
DOI：
10.1093/brain/111.6.1441
复制
发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Harik,SI
中科院分区：
文献类型：
--
作者：
Kalaria,RN;Mitchell,MJ;Harik,SI
在尸检时，在人脑的不同区域，以及同一受试者的脑微血管、脉络丛和肝脏标本中，定量测定了单胺氧化酶(MAO)的两种分子形式MAO-A和MAO-B。通过化学计量的不可逆的特定[~3H]肾上腺素结合和测量几种MAO底物的氧化速率来评估MAO。基底节结构(尾状核、壳核、苍白球和黑质)和海马区MAO含量约为大脑皮层和小脑MAO含量的两倍。构成血脑屏障的脑微血管的MAO含量最低，而构成血脑脊液屏障的脉络丛和肝脏的MAO含量高于任何脑区。这些组织中绝大多数MAO(80%-95%)为B型，但微血管中MAO总活性较低。在所有组织中，特异性的[~3H]pargyline结合与I-甲基-4-苯基-1，2，3，6-四氢吡啶和苯甲胺的氧化速率有很好的相关性。随着年龄的增长，MAO底物的[~3H]肾上腺素结合和氧化速率均增加。
The two molecular forms of monoamine oxidase (MAO), MAO-A and MAO-B, were determined quantitatively in discrete regions of the human brain at autopsy, and in cerebral microvessels, choroid plexus and liver samples from the same subjects. MAO was assessed by specific [3H] pargyline binding, which is stoichiometric and irreversible, and by measuring the rate of oxidation of several MAO substrates. Basal ganglia structures (caudate, putamen, globus pallidus and substantia nigra) and hippocampus had about twice the levels of MAO that were present in the cerebral cortex and cerebellum. Cerebral microvessels, which constitute the blood-brain barrier, had minimal MAO, while the choroid plexus, which constitutes the blood-cerebrospinal fluid barrier, and the liver had higher MAO levels than any brain region. The vast majority of MAO (80–95%) in these tissues was of the B type, except in microvessels, where total MAO activity was low. Specific [3H]pargyline binding correlated well with the oxidation rates for I-methyl-4-phenyl-1,2,3,6-tetrahydropyridine and benzylamine in all tissues. Both specific [3H]pargyline binding and the rate of oxidation of MAO substrates increased with age.