Convection-enhanced delivery of targeted quantum dot-immunoliposome hybrid nanoparticles to intracranial brain tumor models.
Convection-enhanced delivery of targeted quantum dot-immunoliposome hybrid nanoparticles to intracranial brain tumor models.
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DOI：
10.2217/nnm.12.209
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发表时间：
2013-11
期刊：
影响因子：
5.5
通讯作者：
Kevin C Weng;R. Hashizume;C. Noble;L. Serwer;D. Drummond;D. Kirpotin;Anne M. Kuwabara;Lucy X Chao-Lucy-X-C
中科院分区：
文献类型：
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作者：
Kevin C Weng;R. Hashizume;C. Noble;L. Serwer;D. Drummond;D. Kirpotin;Anne M. Kuwabara;Lucy X Chao-Lucy-X-C
目的：通过成像量子点-免疫脂质体杂交纳米颗粒，评估联合靶向策略和对流增强给药在脑肿瘤模型中的作用。材料与方法合成了EGF受体靶向的量子点-免疫脂质体杂化纳米颗粒（QD-IL）。用流式细胞术测定体外摄取，用共聚焦显微镜观察细胞内定位。在体内研究中，通过对流增强递送将qd - il递送到颅内异种移植物中，并实时监测荧光无创。结果qd - il在体外表现出特异性和高效的吸收，在体内与非靶向对应物相比，qd - il在颅内脑肿瘤异种移植物中表现出约1.3- 5.0倍的总荧光。结论：qd - il在体内和体外都是一种有效的显像剂，数据表明，配体定向的纳米粒脂质体结合对流增强给药可能由于恶性细胞特异性和有效的摄取而为胶质母细胞瘤的治疗提供了治疗效果。
AIM The aim of this work is to evaluate combining targeting strategy and convection-enhanced delivery in brain tumor models by imaging quantum dot-immunoliposome hybrid nanoparticles. MATERIALS & METHODS An EGF receptor-targeted, quantum dot-immunoliposome hybrid nanoparticle (QD-IL) was synthesized. In vitro uptake was measured by flow cytometry and intracellular localization was imaged by confocal microscopy. In the in vivo study, QD-ILs were delivered to intracranial xenografts via convection-enhanced delivery and fluorescence was monitored noninvasively in real-time. RESULTS QD-ILs exhibited specific and efficient uptake in vitro and exhibited approximately 1.3- to 5.0-fold higher total fluorescence compared with nontargeted counterpart in intracranial brain tumor xenografts in vivo. CONCLUSION QD-ILs serve as an effective imaging agent in vitro and in vivo, and the data suggest that ligand-directed liposomal nanoparticles in conjunction with convection-enhanced delivery may offer therapeutic benefits for glioblastoma treatment as a result of specific and efficient uptake by malignant cells.