Tissue factor and monocyte chemoattractant protein-1 expression in hypertensive individuals with normal or increased carotid intima-media wall thickness
Tissue factor and monocyte chemoattractant protein-1 expression in hypertensive individuals with normal or increased carotid intima-media wall thickness
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DOI：
10.1373/clinchem.2007.095547
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发表时间：
2008-05-01
影响因子：
9.3
通讯作者：
Saitta, Antonino
中科院分区：
文献类型：
--
作者：
Sardo, Maria A.;Campo, Salvatore;Saitta, Antonino
背景技术背景：高血压患者表现出炎症模式，包括单核细胞趋化蛋白I（MCP-1）和C-反应蛋白（CRP）的血浆浓度增加以及血液单核细胞中组织因子mRNA表达增强。在本研究中，我们研究了CRP浓度与TF和MCP-I mRNA表达之间的关系，从有或没有颈动脉内膜中层厚度（IMT）增加的高血压患者中分离的刺激的单核细胞。我们还研究了TF和MCP-1 mRNA和MCP-1蛋白的表达后，在体外添加CRP的单核细胞。我们测量了80例未经治疗的高血压患者的CRP（通过免疫比浊法）和单核细胞TF和MCP-1的表达（通过实时PCR），与41例对照组相比，这些患者没有临床心血管疾病（CVD）或其他CVD危险因素。根据颈动脉多普勒超声测量的INIT，将患者分为正常（1 mm）的类别。结果：INIT >1 mm的高血压患者CRP、TF和MCP-1 mRNA水平明显高于INIT mm的高血压患者和正常对照组。CRP对高血压患者和对照组单核细胞TF mRNA均无影响。然而，CRP刺激的高血压患者单核细胞显示MCP-1 mRNA和蛋白表达增加。结论：我们的研究结果表明，血液单核细胞的炎症反应在动脉粥样硬化和高血压的发展中起着重要的作用。(c)2008年美国临床化学协会。
BACKGROUND: People with hypertension display an inflammatory pattern that includes increased plasma concentrations of monocyte chemoattractant protein I (MCP-1) and C-reactive protein (CRP) and enhanced expression of tissue factor JF) mRNA in blood monocytes.METHODS: In this study, we investigated the relationship between CRP concentrations and TF and MCP- I mRNA expression in unstimulated and lipopolysaccharide (LPS)-stimulated monocytes isolated from hypertensives with or without an increase in carotid intima-media thickness (IMT). We also investigated the expression of TF and MCP-1 mRNA and MCP-1 protein after in vitro addition of CRP to monocytes. We measured CRP (by immunonephelometry) and monocyte expression of TF and MCP-1 (by real-time PCR) in 80 untreated hypertensive patients without clinical cardiovascular disease (CVD) or additional risk factors for CVD compared with 41 controls. Based on INIT measured by carotid Doppler ultrasonography, patients were classified into the categories of normal (1 mm). TF and MCP-1 mRNA and MCP- I protein (by Western blotting) were measured after in vitro addition of CRP to monocytes from 10 randomized controls as well as 10 hypertensives with IMT 1 mm.RESULTS: CRP and TF and MCP-I mRNA concentrations were significantly higher in INIT >1 mm. hypertensives vs those with INIT mm and controls. CRP had no effect on monocyte TF mRNA from either hypertensives or controls. CRP-stimulated monocytes from hypertensives, however, showed increased MCP-1 mRNA and protein expression compared. with controls and LPS-stimulated cells.CONCLUSIONS: Our findings suggest that the inflammatory response of blood monocytes plays an important role in the development of atherosclerosis and hypertension. (c) 2008 American Association for Clinical Chemistry.