Increased Virus Replication and Cytotoxicity of Non-pathogenic Simian Human Immuno Deficiency Viruses-NM-3rN After Serial Passage in a Monkey-Derived Cell Line.
Increased Virus Replication and Cytotoxicity of Non-pathogenic Simian Human Immuno Deficiency Viruses-NM-3rN After Serial Passage in a Monkey-Derived Cell Line.
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DOI：
10.4103/2141-9248.109490
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发表时间：
2013-01
影响因子：
--
通讯作者：
Miura T
中科院分区：
文献类型：
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作者：
Kwofie T;Miura T
已发现HIV-1变异体在体内的感染和疾病诱导，特别是它们的持续性、复制和疾病进展速率取决于表型特征。然而，这些不同表型特征的机制仍然知之甚少。其目的是确定是否可以使用已适应猴源细胞系的SHIV来解释病毒对其宿主细胞环境的适应性进化的机制。采用病毒学中的标准程序如细胞培养、FACS分析和ELISA来测量病毒复制和生长动力学、细胞活力、逆转录酶（RT）活性测定和CD 4细胞下调。经过大约20次传代后，LT有效地适应了猴源性细胞系，并且比亲本病毒复制得更好。LT在其整个基因组中积累了许多突变，其中大多数是猴细胞特异性的。因此，我们认为我们已经获得了一种病毒，可以通过研究确定这些突变中哪些与体外病毒复制特异性相关，哪些与细胞毒性特异性相关，从而解释与病毒细胞毒性和宿主细胞特异性相关的机制。
Infection and disease induction of variants of HIV type 1 (HIV-1) in vivo, especially their persistence, replication and rate of disease progression, have been found to depend on phenotypic characteristics. However, the mechanism (s) underlying these diverse phenotypic characteristics remain poorly understood. It was aimed at determining whether a SHIV that had been adapted to a monkey-derived cell line could be used to explain the mechanism that underlies adaptive evolution of a virus to its host cell environment. Standard procedures in virology such as cell culturing, FACS analysis and ELISA were employed to measure virus replication and growth kinetics, cell viability, reverse transcriptase (RT) activity assay and CD4 cells down-regulation. After about 20 passages, LT efficiently adapted to the monkey-derived cell line and replicated much better than the parent virus. LT accumulated a number of mutations in its entire genome with a majority of them being monkey cell-specific. Thus we think we have obtained a virus that may enable studies to determine which of these mutations are specifically related to in vitro viral replication and which are specifically related to cytotoxicity so as to explain the mechanism associated with viral cytotoxicity and host cell specificity.