Nearest neighbor thermodynamic parameters for internal G•A mismatches in DNA
Nearest neighbor thermodynamic parameters for internal G•A mismatches in DNA
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DOI：
10.1021/bi9724873
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发表时间：
1998-02-24
期刊：
影响因子：
2.9
通讯作者：
SantaLucia, J
中科院分区：
文献类型：
--
作者：
Allawi, HT;SantaLucia, J
根据吸光度-温度熔化曲线测定了22个内部有单个G.A错配的寡核苷酸在1M氯化钠中溶解的热力学。这些数据结合了五个文献序列。结果表明，G.A错配参数预测Delta G(37)(O)、Delta H-o、Delta S-o和T-M的平均偏差分别为4.4%、7.4%：8.0%和1.5℃。最近邻参数表明G.A错配稳定性强烈依赖于环境，而Delta G(37)(O)；TGA/AAT为+1.16千卡/摩尔，GGC/CAG为-0.78千卡/摩尔。此外，一维H-1核磁共振谱表明，G.A配对，几何构型与pH和上下文有关。
Thermodynamics of 22 oligonucleotides with internal single G.A mismatches dissolved in 1 M NaCl were determined from absorbance versus temperature melting curves. These data, combined with five literature sequences. were used to derive nearest-neighbor thermodynamic parameters for seven linearly independent trimer sequences with internal G.T mismatches and Watson-Crick flanking base pairs, The G.A mismatch parameters predict Delta G(37)(o), Delta H-o, Delta S-o, and T-M With average deviations of 4.4%, 7.4%: 8.0%, and 1.5 degrees C, respectively, The nearest-neighbor parameters show that G.A mismatch stability is strongly context dependent, and Delta G(37)(o); ranges from +1.16 kcal/mol for TGA/AAT to -0.78 kcal/mol for GGC/CAG. In addition, one-dimensional H-1 NMR spectra show that the G.A pairing,geometry is pH and context dependent.