DISTRIBUTION OF SURFACE COAT MATERIAL ON NASAL FOLDS OF MOUSE EMBRYOS AS DEMONSTRATED BY CONCANAVALIN-A BINDING
DISTRIBUTION OF SURFACE COAT MATERIAL ON NASAL FOLDS OF MOUSE EMBRYOS AS DEMONSTRATED BY CONCANAVALIN-A BINDING
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DOI：
10.1002/ar.1091930202
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发表时间：
1979-01-01
影响因子：
--
通讯作者：
LANGMAN, J
中科院分区：
文献类型：
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作者：
BURK, D;SADLER, TW;LANGMAN, J
应用~ 3 H-伴刀豆球蛋白A和伴刀豆球蛋白A-辣根过氧化物酶染色技术，研究了小鼠胚胎鼻襞和鼻沟上皮表面被膜物质的分布。在上皮融合前不久和融合过程中，伴刀豆球蛋白A染色或标记的物质存在于鼻沟和鼻褶上皮细胞的顶面。在鼻沟的限定区域和鼻褶前部和后部区域的假定融合区域中计数代表结合的3 H-伴刀豆球蛋白A的Ag颗粒。对于检查的两个阶段，与前部区域相比，鼻褶后部区域中推定融合区域结合的3 H-伴刀豆球蛋白A的量显著增加;即，在鼻褶之间的接触点之前的区域中表面涂层的量更大。腭架和神经折叠融合的调查表明，增加表面涂层材料的合成与上皮折叠和货架的粘附和融合。
3H-concanavalin A and the concanavalin A-horseradish peroxidase staining technique were used to study the distribution of surface coat material on the epithelium of the nasal folds and nasal groove of mouse embryos. In stages shortly before and during epithelial fusion concanavalin A stained or labeled material was present at apical surfaces of epithelial cells of the nasal groove and nasal folds. Ag grains, representing bound 3H-concanavalin A, were counted in defined areas of the nasal groove and presumptive fusion area in both anterior and posterior regions of the nasal folds. For both stages examined there was a significant increase in the amount of 3H-concanavalin A bound by presumptive fusion areas in posterior regions of the nasal folds as compared with anterior regions; i.e., the amount of surface coat was greater in the region just prior to the point of contact between the nasal folds. Investigations of palatal shelf and neural fold fusion suggest that increased synthesis of surface coat material is associated with adhesion and fusion of epithelial folds and shelves.