Concentration of autoantibodies to native 60-kd Ro/SS-A and denatured 52-kd Ro/SS-A in eluates from the heart of a child who died with congenital complete heart block.
Concentration of autoantibodies to native 60-kd Ro/SS-A and denatured 52-kd Ro/SS-A in eluates from the heart of a child who died with congenital complete heart block.
复制标题
死于先天性完全性心脏传导阻滞的儿童心脏洗脱液中天然 60-kd Ro/SS-A 和变性 52-kd Ro/SS-A 自身抗体的浓度。
DOI：
10.1002/art.1780371120
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发表时间：
1994
影响因子：
--
通讯作者：
Lee,LA
中科院分区：
文献类型：
--
作者：
Reichlin,M;Brucato,A;Frank,MB;Maddison,PJ;McCubbin,VR;Wolfson-Reichlin,M;Lee,LA
Objective.目的探讨1例先天性完全性心脏传导阻滞（CCHB）患儿心脏组织酸洗脱液的血清学特异性。提取组织，酸洗脱，并通过酶联免疫吸附测定法测定洗脱液中的IgG和抗体滴度。天然60-kd和变性52-kd Ro/SS-A的抗体仅在心脏中富集，而在脑、肾和皮肤的洗脱液中不富集。这些发现表明抗天然60-kd Ro/SS-A在CCHB的免疫发病机制中的主要作用。
Objective. To determine the serologic specificity of acid eluates from tissues of a child who died with congenital complete heart block (CCHB).Methods. Tissues were extracted, acid eluted, and the IgG and antibody titers determined on the eluates by enzyme‐linked immunosorbent assay.Results. Antibodies to native 60‐kd and denatured 52‐kd Ro/SS‐A were found to be enriched only in the heart eluate, and not in the eluates from brain, kidney, and skin.Conclusion. These findings indicate a major role for anti–native 60‐kd Ro/SS‐A in the immunopathogenesis of CCHB.