Av-UCP single nucleotide polymorphism affects heat production during cold exposure in chicks.
Av-UCP single nucleotide polymorphism affects heat production during cold exposure in chicks.
复制标题
Av-UCP 单核苷酸多态性影响雏鸡寒冷暴露期间的产热。
DOI：
10.1016/j.jtherbio.2021.102909
复制
发表时间：
2021
期刊：
影响因子：
--
通讯作者：
T.
中科院分区：
文献类型：
--
作者：
Ouchi;Y.;Chowdhury;V.S.;Cockerm;J.F. and Bungo;T.
解偶联蛋白1（UCP 1）参与产热，特别是非颤抖性产热。在鸡中，av-UCP（禽UCP）基因的单核苷酸多态性（SNP）已被报道与体重增加和腹部脂肪增加相关。本研究的目的是探讨鸡av-UCP基因SNP与产热的关系。结果冷暴露后，C/C鸡的平均直肠温度高于T/T鸡;胸肌中av-UCP和carnitine palmitoyltransferase-1基因的表达量在C/C组高于T/T组。促甲状腺激素释放激素和阿黑皮素原基因的表达水平在C/C鸡比T/T鸡高。下丘脑促肾上腺皮质激素释放激素，精氨酸催产素，脑源性神经营养因子和神经肽Y基因的表达没有差异C/C和T/T鸡。此外，血浆游离脂肪酸水平在C/C鸡低于那些T/T chicken.ConclusionThese结果表明，av-UCP基因SNP影响非颤抖性产热通过下丘脑-垂体-甲状腺轴和脂肪酸代谢的鸡。
ObjectiveUncoupling protein one (UCP1) is involved in thermogenesis, especially in non-shivering heat production. In chickens, a single nucleotide polymorphism (SNP) of the av-UCP (avian UCP) gene has been reported to be associated with body weight gain and increased abdominal fat. The purpose of this study was to examine the relationship between the av-UCP gene SNP and heat production in chicks.MethodsC/C and T/T male chicks (Rhode Island Red) of av-UCP gene SNP (g. 1270, C > T) were exposed to a low temperature environment (16 °C for 15 min) and their physiological responses were compared.ResultsAfter cold exposure, mean rectal temperatures of C/C chicks were higher than those of T/T chicks. In pectoral muscle, genes expression of av-UCP and carnitine palmitoyltransferase-1 were higher in C/C chicks than T/T chicks. Hypothalamic expression levels of thyrotropin-releasing hormone and proopiomelanocortin genes were higher in C/C chicks than T/T chicks. Expression of hypothalamic corticotropin-releasing hormone, arginine vasotocin, brain-derived neurotrophic factor and neuropeptide Y genes did not differ between C/C and T/T chicks. In addition, plasma free fatty acid levels in C/C chicks were lower than those of T/T chicks.ConclusionThese results suggest that the av-UCP gene SNP affects non-shivering heat production via the hypothalamo-pituitary-thyroid axis and fatty acid metabolism in the chicken.