Tumor necrosis factor-α induces expression and release of interleukin-6 by human urothelial cells.
Tumor necrosis factor-α induces expression and release of interleukin-6 by human urothelial cells.
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DOI：
10.1007/s00011-010-0298-x
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发表时间：
2011-06
影响因子：
6.7
通讯作者：
Bjorling, Dale E.
中科院分区：
文献类型：
--
作者：
Wang, Zun-Yi;Bjorling, Dale E.
我们检测了肿瘤坏死因子-α (TNF-α)对人尿路上皮细胞（huc）表达和释放白细胞介素-6 （IL-6）的影响，并研究了TNF-α的影响是否通过丝裂原活化蛋白激酶（MAPK）途径介导。用1-10 ng/ml TNF-α处理huc细胞2-24小时。real-time PCR检测IL-6和TNF-α受体1 (TNFR1) mrna的表达。ELISA法测定IL-6在培养基中的释放量。通过免疫印迹分析检测TNFR1蛋白的存在和TNF-α诱导的MAPK通路的激活。测定MAPK通路选择性阻断剂对TNF-α-诱导的IL-6表达和释放的影响。TNF-α以浓度和时间依赖的方式增加IL-6 mRNA的表达并刺激IL-6的释放。TNF-α的作用由TNFR1介导。TNF-α诱导ERK1/2和JNK磷酸化，选择性ERK1/2和JNK阻滞剂抑制TNF-α诱导IL-6的表达和释放。这些结果表明，TNF-α增加huc中IL-6的表达和释放，TNF-α的作用是由TNFR1介导的。此外，ERK1/2和JNK通路参与了huc中TNF-α-诱导的IL-6的表达和释放，可能是炎症性尿路疾病的治疗靶点。
We examined the effects of tumor necrosis factor- α (TNF-α) on expression and release of interleukin-6 (IL-6) by human urothelial cells (HUCs) and investigated whether the effects of TNF-α are mediated by mitogen-activated protein kinase (MAPK) pathways. HUCs were treated with TNF-α at 1–10 ng/ml for 2–24 hours. Expression of IL-6 and TNF-α receptor 1 (TNFR1) mRNAs were examined by real-time PCR. Release of IL-6 into culture medium was determined by ELISA. Presence of TNFR1 protein and TNF-α -induced activation of MAPK pathways was examined by immuoblotting analysis. The effects of selective blockers of MAPK pathway on TNF-α-induced IL-6 expression and release were determined. TNF-α increased IL-6 mRNA expression and stimulated release of IL-6 in a concentration- and time-dependent manner. The effects of TNF-α were mediated by TNFR1. TNF-α induced phosphorylation of the ERK1/2 and JNK, and TNF-α -induced IL-6 expression and release were inhibited by selective ERK1/2 and JNK blockers. These results demonstrate that TNF-α increases expression and release of IL-6 by HUCs and the effects of TNF-α are mediated by TNFR1. Also, the ERK1/2 and JNK pathways are involved in TNF-α-induced expression and release of IL-6 in HUCs and may represent therapeutic targets in inflammatory urinary tract diseases.
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影响因子：
4.8
作者：
Bellei, Barbara;Maresca, Vittoria;Picardo, Mauro
通讯作者：
Picardo, Mauro
影响因子：
4.6
作者：
Malley, SE;Vizzard, MA
通讯作者：
Vizzard, MA
DOI：
10.1152/ajpregu.00570.2006
发表时间：
2007-03-01
影响因子：
2.8
作者：
Qiao, Li-Ya;Gulick, Melisa A.
通讯作者：
Gulick, Melisa A.
影响因子：
7.3
作者：
CUNHA, FQ;POOLE, S;FERREIRA, SH
通讯作者：
FERREIRA, SH
DOI：
10.1152/ajprenal.90733.2008
发表时间：
2009-09-01
影响因子：
4.2
作者：
Tseng-Rogenski, Stephanie;Liebert, Monica
通讯作者：
Liebert, Monica