Detection of Adenovirus in the waters of the Seine River estuary by nested-PCR
Detection of Adenovirus in the waters of the Seine River estuary by nested-PCR
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DOI：
10.1006/mcpr.1998.0166
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发表时间：
1998-06-01
影响因子：
3.3
通讯作者：
Buffet-Janvresse, C
中科院分区：
文献类型：
--
作者：
Castignolles, N;Petit, F;Buffet-Janvresse, C
已经测试了几种从环境样本中分离病毒的系统。最有希望的方法是基于基因组扩增。作者试图通过两步扩增2型腺病毒六邻体蛋白L3保守编码区的220个碱基片段，在塞纳河河口的核酸提取物中检测腺病毒。这项研究中使用的引物检测到了法国人类疾病中最流行的腺病毒血清型，但没有检测到其他病毒株或细菌。套式聚合酶链式反应(PCR)扩增的灵敏度估计为每英里塞纳河水10(2)个拷贝的腺病毒靶序列。对塞纳河河口水域的核酸提取物进行了分析，其中一些提取物的腺病毒检测呈阳性。(C)1998年学术出版社。
Several systems for isolating viruses from environmental samples have been tested. The most promising method is based on genomic amplification. The authors attempted to detect adenovirus in nucleic-acid extracts from the Seine River estuary by a two-step amplification of a 220-bp segment of the conserved coding region of type 2 adenovirus hexon protein L3. The primers used in this study detected the most prevalent adenovirus serotypes in human disease in France, but not other virus strains or bacteria. The sensitivity of the nested polymerase chain reaction (PCR) amplification was estimated to be 10(2) copies of the adenovirus target sequence per mi of Seine River water. Nucleic-acid extracts from Seine River estuary waters were analysed and some tested positive for the presence of adenoviruses. (C) 1998 Academic Press.