Infection of chick chorioallantoic membrane (CAM) as a model for invasive hyphal growth and pathogenesis of Candida albicans
Infection of chick chorioallantoic membrane (CAM) as a model for invasive hyphal growth and pathogenesis of Candida albicans
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DOI：
10.1080/13693780310001600859
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发表时间：
2003-08-01
期刊：
影响因子：
2.9
通讯作者：
Thompson, WD
中科院分区：
文献类型：
--
作者：
Gow, NAR;Knox, Y;Thompson, WD
我们报告了一个简单的模型来评估真菌病原体白色念珠菌入侵受精卵绒毛膜尿囊膜（CAM）的能力。野生型和突变型白色念珠菌以液体悬浮液或无菌滤片的形式接种于CAM表面。膜的侵入导致胚胎因CAM损伤而死亡，这可以通过组织学检查显示细胞分布和形态，并通过RT-PCR评估真菌基因在体内的表达模式。预防或联合接种氟康唑可保护胚胎免受感染。分泌性天冬氨酸蛋白酶（Sap）突变株在CAM模型中具有毒力衰减。然而，两个转录因子Efg1和Cph1突变的白色念珠菌菌株不能在CAM上形成菌丝或穿透它。小企鹅卡姆因此…代表了一种实验上易于处理和廉价的替代啮齿动物或组织培养为基础的入侵模型，并可用于研究真菌的发病机制和遗传调控感染和白色念珠菌的膜渗透。
We report the development of a simple model for assessing the ability of the fungal pathogen Candida albicans to invade the chorioallantoic membrane (CAM) of fertilized hens' eggs. Wild-type and mutant strains of C albicans were inoculated onto CAM surfaces either as a liquid suspension or on a sterile filter disc. Invasion of the membrane led to death of the embryo due to damage of the CAM, which could be examined histologically to show cell distribution and morphology, and by RT-PCR for assessment of patterns of fungal gene expression in vivo. Prophylactic or co-administration of fluconazole with the inoculum protected the embryo from infection. Secretory aspartyl protease (Sap) mutant strains with reported attenuation of virulence were virulent in the CAM model. However, a C. albicans strain with mutations in two transcription factors Efg1 and Cph1 was unable to form hyphae on the CAM or to penetrate it. The chick CAM, therefore.. represents an experimentally tractable and inexpensive alternative to rodent or tissue culture-based invasion models, and can be used to investigate fungal pathogenesis and the genetic regulation of infection and membrane penetration of C albicans.