Characterization of Japanese swine and human hepatitis E virus isolates of genotype IV with 99 % identity over the entire genome
Characterization of Japanese swine and human hepatitis E virus isolates of genotype IV with 99 % identity over the entire genome
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DOI：
10.1099/vir.0.19052-0
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发表时间：
2003-05-01
影响因子：
3.8
通讯作者：
Okamoto, H
中科院分区：
文献类型：
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作者：
Nishizawa, T;Takahashi, M;Okamoto, H
测定了日本猪戊型肝炎病毒（HEV）分离株和人HEV分离株（分别为swJ 13 -1和HE-JA 1）的全长基因组序列，其开放阅读框（ORF）2的部分序列（ORF 2，412 nt）具有100%的同源性。svvJ 13 -1是从2002年出生于日本北海道的4个月大的农场猪中分离的，HE-JA 1是从一名55岁的患者中回收的，该患者居住在北海道，并于1997年感染了散发性急性戊型肝炎。两个分离物均由7240 nt组成（不包括poly（A）尾），含有3个ORF（ORF 1-3），编码1707、674和114 aa的蛋白质。它们的核苷酸序列同源性为99.0%，其中ORF 1-3的氨基酸序列同源性分别为99.8，100和100%。在日本的一个限制性地区观察到的猪和人HEV分离株之间的高度基因组相似性进一步支持了日本散发性戊型肝炎是一种人畜共患病的发现。
The full-length genomic sequences were determined of Japanese swine and human hepatitis E virus (HEV) isolates (swJ13-1 and HE-JA1, respectively) with 100% identity in the partial sequence of open reading frame (ORF) 2 (ORF2, 412 nt). svvJ13-1 was isolated from a 4-month-old farm pig born in Hokkaido, Japan, in 2002 and HE-JA1 was recovered from a 55-year-old patient who lived in Hokkaido and who had contracted sporadic acute hepatitis E in 1997. Both isolates consisted of 7240 nt, excluding the poly(A) tail, and contained three ORFs (ORFs 1-3) that encoded proteins of 1707, 674 and 114 aa. The overall nucleotide sequence identity between them was 99.0% and the deduced amino acid sequence identities of ORFs 1-3 were 99.8, 100 and 100%, respectively. The high degree of genomic similarity observed between swine and human HEV isolates in a restricted area of Japan further supports the finding that sporadic hepatitis E in Japan is a zoonosis.