Comparative immunohistochemical study of endometrioid and serous papillary carcinoma of endometrium.
Comparative immunohistochemical study of endometrioid and serous papillary carcinoma of endometrium.
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发表时间：
2001
影响因子：
0.4
通讯作者：
R. Halperin;S. Zehavi;L. Habler;E. Hadas;I. Bukovsky;D. Schneider
中科院分区：
文献类型：
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作者：
R. Halperin;S. Zehavi;L. Habler;E. Hadas;I. Bukovsky;D. Schneider
目的：本研究的目的是确定是否免疫组化分析的分子参数可以提供一种替代的方法，子宫内膜癌病例的分类，根据其侵略性。方法将64例子宫内膜癌分为3组：Ⅰ组28例为高、中分化（G1-G2）子宫内膜癌，Ⅱ组14例为低分化（G3）子宫内膜癌，Ⅲ组22例为浆液性乳头状子宫内膜癌。采用免疫组化法检测乳腺癌组织中雌激素受体（ER）、孕激素受体（PR）的表达及bcl-2、p53、HER-2/neu和Ki-67的表达。结果：三个研究组的研究分子参数的免疫组化图谱存在显著差异。G1 ~ G2期乳腺癌（I组）ER、PR阳性表达率分别为85.7%、78.6%，bcl-2阳性表达率为42.8%，p53、HER-2/neu低表达率分别为14.3%、14.3%。浆液性乳头状子宫内膜癌（Ⅲ组）ER、PR、bcl-2均为阴性，p53（81.8%）、HER-2/neu（45.4%）为阳性。G3组（Ⅱ组）表现为ER、PR和HER-2/neu免疫阴性，bcl-2免疫阳性率低（7.1%），p53免疫阳性率高（57.1%）。Ki-67在不同子宫内膜癌中的表达差异无统计学意义。结论本研究为子宫内膜癌的免疫组化分析提供了依据，有助于区分不同级别和组织学类型的子宫内膜癌，从而有助于区分高危病例。
OBJECTIVE The aim of this study was to determine whether immunohistochemical analysis of molecular parameters can provide an alternative method for classification of endometrial cancer cases according to their aggressiveness. METHODS Sixty-four cases of endometrial carcinoma were assigned to three groups: group I--28 cases of endometrioid well and moderately differentiated (G1-G2) carcinoma; group II--14 cases of endometrioid poorly differentiated (G3) carcinoma; group III--22 cases of serous papillary endometrial cancer. Immunohistochemistry was used to determine the existence of estrogen receptors (ER), progesterone receptors (PR), and the expression of bcl-2, p53, HER-2/neu and Ki-67. RESULTS There was a significant difference in the immunohistochemical profile of the studied molecular parameters comparing the three study groups. The endometrioid G1-G2 cases (group I) were characterized by increased immunoreactivity for ER and PR (85.7% and 78.6%, respectively), increased immunoreactivity for bcl-2 (42.8%) and low expression of p53 (14.3%) and HER-2/neu (14.3%). In contrast to group I cases, the serous papillary endometrial cancer cases (group III) were characterized by immunonegativity for ER, PR and bcl-2 and high immunoreactivity for p53 (81.8%) and HER-2/neu (45.4%). The endometrioid G3 cases (group II) demonstrated an intermediate immunoprofile, characterized by immunonegativity for ER, PR and HER-2/neu, low immunoreactivity for bcl-2 (7.1%) and high expression of p53 (57.1%). The expression of Ki-67 did not differ significantly comparing the different cases of endometrial cancer. CONCLUSION This study provides evidence that the immunohistochemical analysis of endometrial carcinoma differentiates between different grades and histological types, thus being useful in the distinction of high risk cases.