Genome-wide comparative analysis of putative Pth11-related G protein-coupled receptors in fungi belonging to Pezizomycotina.
Genome-wide comparative analysis of putative Pth11-related G protein-coupled receptors in fungi belonging to Pezizomycotina.
复制标题
Pezizomycotina 真菌中假定的 Pth11 相关 G 蛋白偶联受体的全基因组比较分析
DOI：
10.1186/s12866-017-1076-5
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发表时间：
2017-07-25
期刊：
影响因子：
4.2
通讯作者：
Chen C
中科院分区：
文献类型：
--
作者：
Xu X;Li G;Li L;Su Z;Chen C
研究背景G蛋白偶联受体（GPCRs）是真菌中最大的跨膜受体家族，在信号转导中起重要作用。其中，与Pth 11相关的GPCR形成了一个庞大而分歧的蛋白质家族，并且仅在盘菌亚门的真菌中发现。然而，进化过程和潜在的功能Pth 11相关的GPCR仍然在很大程度上unknow.ResultsTwenty基因组真菌在盘菌亚门涵盖不同的营养策略被挖掘为推定的Pth 11相关的GPCR。植物病原体编码更多的推定Pth 11相关的GPCR比共生体，satellites，或昆虫病原体。基于系统发育树，这些GPCR可分为9个分支，每个分支包含不同分类目的真菌。而不是真菌从同一目的，这些真菌具有相似的营养策略倾向于共享直系同源的假定Pth 11相关的GPCR。大多数含有CFEM结构域的Pth 11相关GPCR仅包含在两个分支中，在植物病原体中检测到。此外，许多植物病原体的Pth 11相关的GPCR基因在入侵植物感染过程中上调，但在生物胁迫下下调。营养条件，特别是碳源，可能会影响卫星和昆虫病原菌的Pth 11相关GPCR基因的表达。Pth 11相关的GPCR基因表达谱分析表明，Pth 11相关的GPCR基因在盘菌亚门分化之前就已存在，并在进化过程中多次获得和/或丢失。串联重复和营养变异是这种进化的重要因素。
BackgroundG-protein coupled receptors (GPCRs) are the largest family of transmembrane receptors in fungi, where they play important roles in signal transduction. Among them, the Pth11-related GPCRs form a large and divergent protein family, and are only found in fungi in Pezizomycotina. However, the evolutionary process and potential functions of Pth11-related GPCRs remain largely unknown.ResultsTwenty genomes of fungi in Pezizomycotina covering different nutritional strategies were mined for putative Pth11-related GPCRs. Phytopathogens encode much more putative Pth11-related GPCRs than symbionts, saprophytes, or entomopathogens. Based on the phylogenetic tree, these GPCRs can be divided into nine clades, with each clade containing fungi in different taxonomic orders. Instead of fungi from the same order, those fungi with similar nutritional strategies were inclined to share orthologs of putative Pth11-related GPCRs. Most of the CFEM domain-containing Pth11-related GPCRs, which were only included in two clades, were detected in phytopathogens. Furthermore, many putative Pth11-related GPCR genes of phytopathogens were upregulated during invasive plant infection, but downregulated under biotic stress. The expressions of putative Pth11-related GPCR genes of saprophytes and entomopathogens could be affected by nutrient conditions, especially the carbon source. The gene expressions revealed that Pth11-related GPCRs could respond to biotic/abiotic stress and invasive plant infection with different expression patterns.ConclusionOur results indicated that the Pth11-related GPCRs existed before the diversification of Pezizomycotina and have been gained and/or lost several times during the evolutionary process. Tandem duplications and trophic variations have been important factors in this evolution.