Modulation of skin collagen metabolism in aged and photoaged human skin in vivo
Modulation of skin collagen metabolism in aged and photoaged human skin in vivo
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DOI：
10.1046/j.0022-202x.2001.01544.x
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发表时间：
2001-11-01
影响因子：
6.5
通讯作者：
Eun, HC
中科院分区：
文献类型：
--
作者：
Chung, JH;Seo, JY;Eun, HC
据我们所知，迄今为止还没有进行过直接比较光老化和自然老化人类皮肤的胶原蛋白代谢的研究。在这项研究中，我们比较了胶原合成、基质金属蛋白酶-1水平和明胶酶活性的日晒和防晒的年轻和老年受试者。通过northern blot分析、免疫组织化学染色和Western blot分析，我们发现光老化和自然老化的人体皮肤中I型前胶原mRNA和蛋白的水平明显低于年轻皮肤。此外，我们通过northern blot分析证明，在同一个体中，光老化皮肤的前胶原α 1(I) mRNA表达量远高于防晒皮肤。原位杂交和免疫组织化学染色显示，光老化皮肤成纤维细胞中I型前胶原mRNA和蛋白的表达高于自然老化皮肤。此外，用真皮组织提取蛋白进行Western blot分析发现，光老化皮肤中I型前胶原蛋白的水平低于自然老化皮肤。光老化皮肤真皮中基质金属蛋白酶-1蛋白水平和基质金属蛋白酶-2活性均高于自然老化皮肤。我们的研究结果表明，自然衰老过程减少了胶原合成并增加了基质金属蛋白酶的表达，而光老化导致体内人体皮肤中胶原合成增加和基质金属蛋白酶的表达增加。因此，在光老化和自然老化的皮肤中，胶原蛋白合成和降解之间的平衡导致胶原蛋白缺乏是不同的。
To the best of our knowledge, no study has been conducted to date to directly compare the collagen metabolism of photoaged and naturally aged human skin. In this study, we compared collagen synthesis, matrix metalloproteinase-1 levels, and gelatinase activity of sun-exposed and sun-protected skin of both young and old subjects. Using northern blot analysis, immunohistochemical stain, and Western blot analysis, we demonstrated that the levels of procollagen type I mRNA and protein in photoaged and naturally aged human skin in vivo are significantly lower than those of young skin. Furthermore, we demonstrated, by northern blot analysis, that the procollagen alpha1(I) mRNA expression of photoaged skin is much greater than that of sun-protected skin in the same individual. In situ hybridization and immunohistochemical stain were used to show that the expression of type I procollagen mRNA and protein in the fibroblasts of photoaged skin is greater than for naturally aged skin. In addition, it was found, by Western blot analysis using protein extracted from the dermal tissues, that the level of procollagen type I protein in photoaged skin is lower than that of naturally aged skin. The level of matrix metalloproteinase-1 protein and the activity of matrix metalloproteinase-2 were higher in the dermis of photoaged skin than in naturally aged skin. Our results suggest that the natural aging process decreases collagen synthesis and increases the expression of matrix metalloproteinases, whereas photoaging results in an increase of collagen synthesis and greater matrix metalloproteinase expression in human skin in vivo. Thus, the balance between collagen synthesis and degradation leading to collagen deficiency is different in photoaged and naturally aged skin.